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Gene Expression Patterns during the Early Stages of Chemically Induced Larval Metamorphosis and Settlement of the Coral Acropora millepora

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dc.contributor Sch Biol Earth & Environm Sci
dc.contributor University Of New South Wales Sydney
dc.contributor Ctr Marine Bioinnovat
dc.contributor Univ New S Wales
dc.contributor Australian Inst Marine Sci
dc.contributor Australian Institute Of Marine Science
dc.contributor.author HARDER, TILMANN
dc.contributor.author SIBONI, NACHSHON
dc.contributor.author ABREGO, DAVID
dc.contributor.author MOTTI, CHERIE A.
dc.contributor.author TEBBEN, JAN
dc.date.accessioned 2014-05-09T04:24:11Z
dc.date.accessioned 2014-05-09T04:24:11Z
dc.date.accessioned 2017-03-21T01:08:30Z
dc.date.accessioned 2019-05-09T01:03:22Z
dc.date.available 2017-03-21T01:08:30Z
dc.date.available 2017-03-21T01:08:30Z
dc.date.available 2014-05-09T04:24:11Z
dc.date.available 2019-05-09T01:03:22Z
dc.date.issued 2014-03-14
dc.identifier.citation Siboni N, Abrego D, Motti CA, Tebben J, Harder T (2014) Gene expression patterns during the early stages of chemically induced larval metamorphosis and settlement of the coral Acropora millepora. PLoS ONE 9(3): e91082 en_US
dc.identifier.issn 1932-6203
dc.identifier.uri http://epubs.aims.gov.au/11068/10361
dc.description.abstract The morphogenetic transition of motile coral larvae into sessile primary polyps is triggered and genetically programmed upon exposure to environmental biomaterials, such as crustose coralline algae (CCA) and bacterial biofilms. Although the specific chemical cues that trigger coral larval morphogenesis are poorly understood there is much more information available on the genes that play a role in this early life phase. Putative chemical cues from natural biomaterials yielded defined chemical samples that triggered different morphogenetic outcomes: an extract derived from a CCA-associated Pseudoalteromonas bacterium that induced metamorphosis, characterized by non-attached metamorphosed juveniles; and two fractions of the CCA Hydrolithon onkodes (Heydrich) that induced settlement, characterized by attached metamorphosed juveniles. In an effort to distinguish the genes involved in these two morphogenetic transitions, competent larvae of the coral Acropora millepora were exposed to these predictable cues and the expression profiles of 47 coral genes of interest (GOI) were investigated after only 1 hour of exposure using multiplex RT-qPCR. Thirty-two GOI were differentially expressed, indicating a putative role during the early regulation of morphogenesis. The most striking differences were observed for immunity-related genes, hypothesized to be involved in cell recognition and adhesion, and for fluorescent protein genes. Principal component analysis of gene expression profiles resulted in separation between the different morphogenetic cues and exposure times, and not only identified those genes involved in the early response but also those which influenced downstream biological changes leading to larval metamorphosis or settlement.
dc.description.sponsorship Funding was provided by the Australian Institute of Marine Science, Futures Project, Appropriation Fund 2233. TH was partially supported by a Research Fellowship awarded by the German Research Foundation (HA 3496/5-1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.description.sponsorship Funding was provided by the Australian Institute of Marine Science, Futures Project, Appropriation Fund 2233 en_US
dc.description.uri http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0091082 en_US
dc.language English
dc.language.iso en en_US
dc.publisher PLos Open Access en_US
dc.relation.ispartof Null
dc.rights Attribution 3.0 Australia *
dc.rights.uri http://creativecommons.org/licenses/by/3.0/au/ *
dc.subject Immunity
dc.subject Algae
dc.subject Science & Technology - Other Topics
dc.subject Multidisciplinary Sciences
dc.subject Protein
dc.title Gene Expression Patterns during the Early Stages of Chemically Induced Larval Metamorphosis and Settlement of the Coral Acropora millepora
dc.type journal article en_US
dc.identifier.doi 10.1371/journal.pone.0091082
dc.identifier.wos WOS:000332858400039


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