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Multiomics analysis of the giant triton snail salivary gland, a crown-of-thorns starfish predator

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dc.contributor Genecol Res Ctr
dc.contributor Fac Sci Hlth Educ & Engn
dc.contributor University Of The Sunshine Coast
dc.contributor Australian Inst Marine Sci
dc.contributor Univ Sunshine Coast
dc.contributor Australian Institute Of Marine Science CUMMINS, S. F. BOSE, U. WANG, T. ZHAO, M. MOTTI, C. A. HALL, M. R. 2017-10-01T18:22:38Z 2017-10-01T18:22:38Z 2019-05-09T01:04:25Z 2017-10-01T18:22:38Z 2017-10-01T18:22:38Z 2019-05-09T01:04:25Z 2017-07-20
dc.identifier.citation Bose U, Wang T, Zhao M, Motti CA, Hall MR, Cummins SF (2017) Multiomics analysis of the giant triton snail salivary gland, a crown-of-thorns starfish predator. Scientific Reports 7: 6000
dc.identifier.issn 2045-2322
dc.description.abstract The giant triton snail (Charonia tritonis) is one of the few natural predators of the adult Crown-of-Thorns starfish (COTS), a corallivore that has been damaging to many reefs in the Indo-Pacific. Charonia species have large salivary glands (SGs) that are suspected to produce either a venom and/or sulphuric acid which can immobilize their prey and neutralize the intrinsic toxic properties of COTS. To date, there is little information on the types of toxins produced by tritons. In this paper, the predatory behaviour of the C. tritonis is described. Then, the C. tritonis SG, which itself is made up of an anterior lobe (AL) and posterior lobe (PL), was analyzed using an integrated transcriptomics and proteomics approach, to identify putative toxin-and feeding-related proteins. A de novo transcriptome database and in silico protein analysis predicts that similar to 3800 proteins have features consistent with being secreted. A gland-specific proteomics analysis confirmed the presence of numerous SG-AL and SG-PL proteins, including those with similarity to cysteine-rich venom proteins. Sulfuric acid biosynthesis enzymes were identified, specific to the SG-PL. Our analysis of the C. tritonis SG (AL and PL) has provided a deeper insight into the biomolecular toolkit used for predation and feeding by C. tritonis.
dc.description.sponsorship This work was supported by grants from the Australian federal government Department of the Environment Reef Rescue 'Caring for Country' program (MRH, SFC, Project ID A0000010389G). This research was undertaken with the assistance of resources provided from the Marine Research Center at Kavieng, PNG, and the NCI National Facility systems at the Australian National University through the National Computational Merit Allocation Scheme supported by the Australian Government. We thank Varvara Barkalova for her advice on C. tritonis anatomy. Further thanks go to Peter Thomas-Hall for assistance in the husbandry of giant triton held at the AIMS SeaSim precinct and for filming giant tritons attacking COTS.
dc.language English
dc.subject Acanthaster-planci
dc.subject Gastropod Monoplex-echo
dc.subject Science & Technology - Other Topics
dc.subject Rich Secretory Proteins
dc.subject Crystal-structure
dc.subject Aminopeptidase-n
dc.subject Signal Peptides
dc.subject Multidisciplinary Sciences
dc.subject Cymatium-intermedius
dc.subject Foregut Glands
dc.subject Saponin-detoxifying Enzyme
dc.subject Marine Gastropod
dc.title Multiomics analysis of the giant triton snail salivary gland, a crown-of-thorns starfish predator
dc.type journal article
dc.identifier.doi 10.1038/s41598-017-05974-x
dc.identifier.wos WOS:000405907800054

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