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Comparative study of the toxic effects of Chrysaora quinquecirrha (Cnidaria: Scyphozoa) and Chironex fleckeri (Cnidaria: Cubozoa) venoms using cell-based assays

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dc.contributor Australian Institute Of Marine Science
dc.contributor Univ Tasmania
dc.contributor Dept Pharmacol & Therapeut
dc.contributor Townsville Mail Ctr
dc.contributor Cardiovasc Therapeut Unit
dc.contributor Australian Venom Res Unit
dc.contributor Univ Melbourne
dc.contributor University Of Tasmania
dc.contributor Australian Inst Marine Sci
dc.contributor University Of Melbourne
dc.contributor Inst Marine & Antarctic Studies
dc.contributor.author DORANTES-ARANDA, JUAN JOSE
dc.contributor.author PONCE, DALIA
dc.contributor.author BRINKMAN, DIANE L.
dc.contributor.author LUNA-RAMIREZ, KAREN
dc.contributor.author WRIGHT, CHRISTINE E.
dc.date.accessioned 2015-10-06T05:38:12Z
dc.date.accessioned 2015-10-06T05:38:12Z
dc.date.accessioned 2017-03-21T01:02:16Z
dc.date.accessioned 2019-07-08T02:21:24Z
dc.date.available 2017-03-21T01:02:16Z
dc.date.available 2017-03-21T01:02:16Z
dc.date.available 2015-10-06T05:38:12Z
dc.date.available 2019-07-08T02:21:24Z
dc.date.issued 2015-11-01
dc.identifier.citation Ponce D, Brinkman DL, Luna-Ramirez K, Wright CE, Dorantes-Aranda JJ (2015) Comparative study of the toxic effects of Chrysaora quinquecirrha (Cnidaria: Scyphozoa) and Chironex fleckeri (Cnidaria: Cubozoa) venoms using cell-based assays. Toxicon 106: 57-67 en_US
dc.identifier.issn 0041-0101
dc.identifier.uri http://epubs.aims.gov.au/11068/11361
dc.description.abstract The venoms of jellyfish cause toxic effects in diverse biological systems that can trigger local and systemic reactions. In this study, the cytotoxic and cytolytic effects of Chrysaora quinquecirrha and Chironex flecked venoms were assessed and compared using three in vitro assays. Venoms from both species were cytotoxic to fish gill cells and rat cardiomyocytes, and cytolytic in sheep erythrocytes. Both venoms decreased cell viability in a concentration-dependent manner; however, the greatest difference in venom potencies was observed in the fish gill cell line, wherein C. fleckeri was 12.2- (P = 0.0005) and 35.7-fold (P < 0.0001) more potently cytotoxic than C. quinquecirrha venom with 30 min and 120 min cell exposure periods, respectively. Gill cells and rat cardiomyocytes exposed to venoms showed morphological changes characterised by cell shrinkage, clumping and detachment. The cytotoxic effects of venoms may be caused by a group of toxic proteins that have been previously identified in C fleckeri and other cubozoan jellyfish species. In this study, proteins homologous to CfTX-1 and CfFX-2 toxins from C. fleckeri and CqTX-A toxin from Chironex yamaguchii were identified in C quinquecirrha venom using tandem mass spectrometry. The presence and relative abundance of these proteins may explain the differences in venom potency between cubozoan and scyphozoan jellyfish and may reflect their importance in the action of venoms. (C) 2015 Elsevier Ltd. All rights reserved.
dc.description.sponsorship This work was supported by the Melbourne International Fee Remission Scholarship (MIFRS) and Melbourne International Research Scholarship (MIRS) from the University of Melbourne and by the Scholarship for Postgraduate Studies from the National Council of Science and Technology from Mexico (CONACyT) awarded to D. P. We thank The Struan Sutherland Trust funding to the Australian Venom Research Unit (AVRU) for supporting K. L-R. and D. P. The authors also thank the Australian Research Council, grant DP130102859, for funding J.J.D-A. We-thank Senior Aquarist Sharyl Crossley from the Tennessee Aquarium for her kind assistance in sampling and processing of C. quinquecirrha jellyfish specimens. The authors also acknowledge Dr. Ken Winkel from AVRU (University of Melbourne) for his comments to the manuscript and Dr Jon Mangum from the Laboratory of Proteomics and Calcium (University of Melbourne) for his help in sample processing, protein identifications and comments to improve the manuscript. We also thank David Ian Stapleton from The Florey Institute of Neuroscience and Mental Health for sample processing and protein identifications at Bio21 Institute (University of Melbourne).
dc.description.uri 10.1016/j.toxicon.2015.09.014 en_US
dc.language English
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.relation.ispartof Null
dc.subject Protein Toxin
dc.subject Chironex Fleckeri
dc.subject Fishing Tentacle
dc.subject Stomolophus-meleagris
dc.subject Chrysaora Quinquecirrha
dc.subject Cyanea-capillata
dc.subject Habu-kurage
dc.subject Partial-purification
dc.subject War Physalia-physalis
dc.subject Australian Box Jellyfish
dc.subject Nematocyst Venom
dc.subject Sea Nettle Toxin
dc.subject Pharmacology & Pharmacy
dc.subject Toxicology
dc.subject Cell Line en_US
dc.subject Venom en_US
dc.subject Jellyfish en_US
dc.subject Cftx en_US
dc.title Comparative study of the toxic effects of Chrysaora quinquecirrha (Cnidaria: Scyphozoa) and Chironex fleckeri (Cnidaria: Cubozoa) venoms using cell-based assays
dc.type journal article en_US
dc.identifier.doi 10.1016/j.toxicon.2015.09.014
dc.identifier.wos WOS:000364252400008


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