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The sponge microbiome project

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dc.contributor Dept Life & Environm Sci
dc.contributor Australian Ctr Ecogen
dc.contributor Univ New South Wales
dc.contributor Tel Aviv University
dc.contributor State University of New York (SUNY) Stony Brook
dc.contributor University of Auckland
dc.contributor University of Haifa
dc.contributor University of California System
dc.contributor Halmos Coll Nat Sci & Oceanog
dc.contributor Sch Biol Sci
dc.contributor Hellenic Centre for Marine Research
dc.contributor George S Wise Fac Life Sci
dc.contributor University of Alabama System
dc.contributor CMAEE
dc.contributor Univ Calif San Diego
dc.contributor Natl Univ Ireland Galway
dc.contributor Universite de Toulouse
dc.contributor Dept Ecol & Evolut
dc.contributor Lab Microbiol
dc.contributor Universidade Tecnica de Lisboa
dc.contributor Hellen Ctr Marine Res
dc.contributor Dept Pediat
dc.contributor Institute of Marine & Environmental Technology
dc.contributor Christian Albrechts Univ Kiel
dc.contributor Ctr Marine Bioinnovat
dc.contributor Dept Bioengn
dc.contributor UMR1309
dc.contributor University of Notre Dame
dc.contributor Cirad
dc.contributor University of North Carolina
dc.contributor Wageningen Univ
dc.contributor GEOMAR Helmholtz Ctr Ocean Res
dc.contributor University System of Maryland
dc.contributor Universidade de Lisboa
dc.contributor Univ Queensland
dc.contributor Universite Toulouse III - Paul Sabatier
dc.contributor CIRAD
dc.contributor University of North Carolina Wilmington
dc.contributor State University of New York (SUNY) System
dc.contributor Sch Biol Earth & Environm Sci
dc.contributor Univ Notre Dame
dc.contributor Dept Zool
dc.contributor Inst Chem & Biol Marine Environm ICBM
dc.contributor Dept Microbiol & Immunol
dc.contributor Dept Marine Biol
dc.contributor Ctr Microbiome Innovat
dc.contributor Tel Aviv Univ
dc.contributor University of Queensland
dc.contributor Shanghai Jiao Tong Univ
dc.contributor Div Biol & Environm Sci & Engn
dc.contributor Victoria University Wellington
dc.contributor Shanghai Jiao Tong University
dc.contributor Dept Biol Sci
dc.contributor Univ Auckland
dc.contributor National University of Ireland (NUI) Galway
dc.contributor Nova Southeastern University
dc.contributor State Key Lab Microbial Metab
dc.contributor Dept Biol & Marine Biol
dc.contributor Univ Lisbon
dc.contributor University of British Columbia
dc.contributor Galvin Life Sci Ctr
dc.contributor King Abdullah Univ Sci & Technol
dc.contributor Univ North Carolina Wilmington
dc.contributor Polytech Univ Marche
dc.contributor University of Maryland Center for Environmental Science
dc.contributor University of Kiel
dc.contributor UMR15
dc.contributor KEEP
dc.contributor Univ British Columbia
dc.contributor Marche Polytechnic University
dc.contributor Univ Maryland
dc.contributor AIMS
dc.contributor Inst Marine & Environm Technol
dc.contributor Leon H Charney Sch Marine Sci
dc.contributor Nova Southeastern Univ
dc.contributor Sch Nat Sci
dc.contributor University of Maryland Baltimore
dc.contributor Centre National de la Recherche Scientifique (CNRS)
dc.contributor Sch Life Sci & Biotechnol
dc.contributor Carl von Ossietzky Universitat Oldenburg
dc.contributor Dept Comp Sci & Engn
dc.contributor INRA
dc.contributor Institut National de la Recherche Agronomique (INRA)
dc.contributor Ctr Environm Sci
dc.contributor SUNY Stony Brook
dc.contributor Carl von Ossietzky Univ Oldenburg
dc.contributor University of New South Wales Sydney
dc.contributor RD3 Marine Microbiol
dc.contributor CNRS
dc.contributor University of California San Diego
dc.contributor Ecol Networks & Global Change Grp
dc.contributor Paul Sabatier Univ
dc.contributor Zool
dc.contributor Theoret & Expt Ecol Stn
dc.contributor Inst Marine Biol Biotechnol & Aquaculture
dc.contributor Univ Haifa
dc.contributor Victoria Univ Wellington
dc.contributor King Abdullah University of Science & Technology
dc.contributor Universite Federale Toulouse Midi-Pyrenees (ComUE)
dc.contributor IBB
dc.contributor Hawaii Inst Marine Biol
dc.contributor University of Alabama Tuscaloosa
dc.contributor Wageningen University & Research
dc.contributor IST
dc.contributor Ryan Inst
dc.contributor Univ Alabama
dc.contributor Sch Chem & Mol Biosci Reveillaud, Julie Nielsen, Shaun Marra, Maria V. Feng, Guofang Dailianis, Thanos Lopez-Legentil, Susanna Easson, Cole Li, Zhiyong Steinert, Georg Ackermann, Gail L. Schupp, Peter J. Webster, Nicole S. Ilan, Micha Moitinho-Silva, Lucas Polymenakou, Paraskevi Erwin, Patrick M. Hill, Russell T. Liu, Fang Bjork, Johannes R. Amir, Amnon Simister, Rachel L. Vicente, Jan Knight, Rob Montoya, Jose M. Bell, James J. Taylor, Michael W. Cerrano, Carlo Sipkema, Detmer Thomas, Torsten Gonzalez, Antonio Astudillo-Garcia, Carmen Thacker, Robert W. Olson, Julie B. Costa, Rodrigo Kotoulas, Giorgos Ravasi, Timothy McCormack, Grace P. Hentschel, Ute Pineda, Mari-Carmen Steindler, Laura 2017-10-22T19:05:37Z 2017-11-15T03:10:18Z 2017-10-22T19:05:37Z 2017-11-15T03:10:18Z 2017-10-01
dc.identifier.citation Moitinho-Silva L, Nielsen S, Amir A, Gonzalez A, Ackermann GL, Cerrano C, Astudillo-Garcia C, Easson C, Sipkema D, Liu F, Steinert G, Kotoulas G, McCormack GP, Feng GF, Bell JJ, Vicente J, Bjork JR, Montoya JM, Olson JB, Reveillaud J, Steindler L, Pineda MC, Marra MV, Ilan M, Taylor MW, Polymenakou P, Erwin PM, Schupp PJ, Simister RL, Knight R, Thacker RW, Costa R, Hill RT, Lopez-Legentil S, Dailianis T, Ravasi T, Hentschel U, Li ZY, Webster NS, Thomas T (2017) The sponge microbiome project. GigaScience 6(10): 1-7
dc.identifier.issn 2047-217X
dc.description.abstract Marine sponges (phylum Porifera) are a diverse, phylogenetically deep-branching clade known for forming intimate partnerships with complex communities of microorganisms. To date, 16S rRNA gene sequencing studies have largely utilised different extraction and amplification methodologies to target the microbial communities of a limited number of sponge species, severely limiting comparative analyses of sponge microbial diversity and structure. Here, we provide an extensive and standardised dataset that will facilitate sponge microbiome comparisons across large spatial, temporal, and environmental scales. Samples from marine sponges (n = 3569 specimens), seawater (n = 370), marine sediments (n = 65) and other environments (n = 29) were collected from different locations across the globe. This dataset incorporates at least 268 different sponge species, including several yet unidentified taxa. The V4 region of the 16S rRNA gene was amplified and sequenced from extracted DNA using standardised procedures. Raw sequences (total of 1.1 billion sequences) were processed and clustered with (i) a standard protocol using QIIME closed-reference picking resulting in 39 543 operational taxonomic units (OTU) at 97% sequence identity, (ii) a de novo clustering using Mothur resulting in 518 246 OTUs, and (iii) a new high-resolution Deblur protocol resulting in 83 908 unique bacterial sequences. Abundance tables, representative sequences, taxonomic classifications, and metadata are provided. This dataset represents a comprehensive resource of sponge-associated microbial communities based on 16S rRNA gene sequences that can be used to address overarching hypotheses regarding host-associated prokaryotes, including host specificity, convergent evolution, environmental drivers of microbiome structure, and the sponge-associated rare biosphere.
dc.description.sponsorship T.T. and N.S.W were funded by Australian Research Council Future Fellowships FT140100197 and FT120100480, respectively. T.T. received funds from the Gordon and Betty Moore Foundation. This work was also supported in part by the W.M. Keck Foundation and the John Templeton Foundation. R.K. received funding as a Howard Hughes Medical Institute Early Career Scientist.
dc.language English
dc.subject REVEALS
dc.subject microbial diversity
dc.subject EVOLUTION
dc.subject microbiome
dc.subject Science & Technology - Other Topics
dc.subject Multidisciplinary Sciences
dc.subject symbiosis
dc.subject marine sponges
dc.subject 16S rRNA gene
dc.subject SILVA
dc.subject archaea
dc.subject bacteria
dc.subject DIVERSITY
dc.subject TOOLS
dc.title The sponge microbiome project
dc.type journal article
dc.identifier.doi 10.1093/gigascience/gix077
dc.identifier.wos WOS:000412397500002

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