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Producing Coral Offspring with Cryopreserved Sperm: A Tool for Coral Reef Restoration

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dc.contributor Hawaii Inst Marine Biol
dc.contributor Ctr Species Survival
dc.contributor Smithsonian Institution
dc.contributor Smithsonian Inst
dc.contributor Taronga Conservat Soc Australia
dc.contributor Univ Melbourne
dc.contributor Australian Inst Marine Sci
dc.contributor Univ Hawaii
dc.contributor University Of Melbourne
dc.contributor Smithsonian Conservat Biol Inst
dc.contributor University Of Hawaii System
dc.contributor Australian Institute Of Marine Science
dc.contributor Sch Biosci
dc.contributor.author SPINDLER, REBECCA E.
dc.contributor.author HAGEDORN, MARY
dc.contributor.author CARTER, VIRGINIA L.
dc.contributor.author HENLEY, E. MICHAEL
dc.contributor.author VAN OPPEN, MADELEINE J. H.
dc.contributor.author HOBBS, REBECCA
dc.date.accessioned 2017-11-19T18:55:21Z
dc.date.accessioned 2017-11-19T18:55:21Z
dc.date.accessioned 2019-07-08T02:08:28Z
dc.date.available 2017-11-19T18:55:21Z
dc.date.available 2017-11-19T18:55:21Z
dc.date.available 2019-07-08T02:08:28Z
dc.date.issued 2017-10-31
dc.identifier.citation Hagedorn M, Carter VL, Henley EM, van Oppen MJH, Hobbs R, Spindler RE (2017) Producing coral offspring with cryopreserved sperm: A tool for coral reef restoration. Scientific Reports 7: 14432
dc.identifier.issn 2045-2322
dc.identifier.uri http://epubs.aims.gov.au/11068/11822
dc.description.abstract Cryopreservation is an important conservation tool, which may help reef-building coral survive. However, scaling-up from small, laboratory-sized experiments to higher-throughput restoration is a major challenge. To be an effective restoration tool, the cryopreservation methods and husbandry to produce new offspring must be defined. This study examined small and larger-scale in vitro reproduction and settlement for Acropora tenuis and Acropora millepora and found that: 1) cryopreservation of coral sperm reduced sperm motility and fertilization success in half, thus fresh sperm, capable of becoming highly motile, is key; 2) the sperm-to-egg ratio and the concentration of the cryoprotectant treatments affected fertilization success in small-and larger-scale reproduction trials using cryopreserved sperm (p < 0.05); 3) cryopreservation did not affect settlement success, as larvae produced with fresh or cryopreserved sperm had the same settlement success (p > 0.05); and 4) the residence time of the sperm within the bank was not important as the fertilization success of sperm frozen for less than 1 month was similar to that frozen up to 2 years (p > 0.05). These results described the first settlement for coral larvae produced from cryopreserved sperm and established important ground-work principles for the use of cryopreserved coral sperm for future reef restoration efforts.
dc.description.sponsorship We would like to thank the following people from the Australian Institute of Marine Science and the National Sea Simulator at the Australian Institute of Marine Science for the help and collaboration: Andrew Negri, Craig Humphrey, Andrea Severati, Lina Bay, Bette Willis, David Abrego, Emily Howells and Martina Zarri for counting our selected settlers at the end of 2014. These experiments were supported through funds from the Smithsonian Institution, the Hawaii Institute of Marine Biology, the Australian Institute of Marine Science, the Taronga Conservation Society Australia, the Roddenberry Foundation, the Matthew Frank Foundation, the Anela Kolohe Foundation, and the Cedarhill Foundation to MH. This manuscript was approved for publication by the Hawaii Institute of Marine Biology as contribution # 1703.
dc.language English
dc.subject Spermatozoa
dc.subject Resilience
dc.subject Science & Technology - Other Topics
dc.subject Impacts
dc.subject Future
dc.subject Multidisciplinary Sciences
dc.subject Climate-change
dc.subject Recovery
dc.title Producing Coral Offspring with Cryopreserved Sperm: A Tool for Coral Reef Restoration
dc.type journal article
dc.identifier.doi 10.1038/s41598-017-14644-x
dc.identifier.wos WOS:000414231000033


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