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Global transcriptomic profiling in barramundi (Lates calcarifer) from rivers impacted by differing agricultural land uses

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dc.contributor Australian Institute Of Marine Science
dc.contributor Queensland Dept Sci Informat Technol & Innovat
dc.contributor Queensland University Of Technology (qut)
dc.contributor Commonwealth Scientific & Industrial Research Organisation (csiro)
dc.contributor Australian Inst Marine Sci
dc.contributor Csiro
dc.contributor.author WESTCOTT, DAVID A.
dc.contributor.author HOOK, SHARON E.
dc.contributor.author KROON, FREDERIEKE J.
dc.contributor.author METCALFE, SUZANNE
dc.contributor.author GREENFIELD, PAUL A.
dc.contributor.author MONCUQUET, PHILIPPE
dc.contributor.author MCGRATH, ANNETTE
dc.contributor.author SMITH, RACHAEL
dc.contributor.author WARNE, MICHAEL ST. J.
dc.contributor.author TURNER, RYAN D.
dc.contributor.author MCKEOWN, ADAM
dc.date.accessioned 2017-03-21T01:03:52Z
dc.date.accessioned 2017-03-21T01:03:52Z
dc.date.accessioned 2017-01-13T00:42:51Z
dc.date.accessioned 2019-07-08T02:11:20Z
dc.date.available 2017-01-13T00:42:51Z
dc.date.available 2017-01-13T00:42:51Z
dc.date.available 2017-03-21T01:03:52Z
dc.date.available 2019-07-08T02:11:20Z
dc.date.issued 2017-01-01
dc.identifier.citation Hook SE, Kroon FJ, Metcalfe S, Greenfield PA, Moncuquet P, McGrath A, Smith R, Warne MS, Turner RD, McKeown A, Westcott DA (2017) Global transcriptomic profiling in barramundi (Lates calcarifer) from rivers impacted by differing agricultural land uses. Environmental Toxicology and Chemistry 36(1): 103-112 en_US
dc.identifier.issn 0730-7268
dc.identifier.uri http://epubs.aims.gov.au/11068/13140
dc.description.abstract Most catchments discharging into the Great Barrier Reef lagoon have elevated loads of suspended sediment, nutrients, and pesticides, including photosystem II inhibiting herbicides, associated with upstream agricultural land use. To investigate potential impacts of declining water quality on fish physiology, RNA sequencing (RNASeq) was used to characterize and compare the hepatic transcriptomes of barramundi (Lates calcarifer) captured from 2 of these tropical river catchments in Queensland, Australia. The Daintree and Tully Rivers differ in upstream land uses, as well as sediment, nutrient, and pesticide loads, with the area of agricultural land use and contaminant loads lower in the Daintree. In fish collected from the Tully River, transcripts involved in fatty acid metabolism, amino acid metabolism, and citrate cycling were also more abundant, suggesting elevated circulating cortisol concentrations, whereas transcripts involved in immune responses were less abundant. Fish from the Tully also had an increased abundance of transcripts associated with xenobiotic metabolism. Previous laboratory-based studies observed similar patterns in fish and amphibians exposed to the agricultural herbicide atrazine. If these transcriptomic patterns are manifested at the whole organism level, the differences in water quality between the 2 rivers may alter fish growth and fitness. Environ Toxicol Chem 2017;36:103-112. (c) 2016 SETAC
dc.description.sponsorship Funding for the analyses of the fish samples was provided to SEH by CSIRO Oceans and Atmosphere; computational support was provided by the bioinformatics core at CSIRO Computation Informatics; sequencing was provided by the Ramaciotti Centre for Gene Function Analysis at the University of New South Wales. en_US
dc.description.sponsorship The field collections were conducted when F.J. Kroon was employed by the Commonwealth Scientific and Industrial Research Organisation (CSIRO), with permission from the Queensland Department of Agriculture, Fisheries, and Forestry, and the CSIRO Ecosystem Sciences' Animal Ethics Committee, and were funded by CSIRO's Water for a Healthy Country Flagship Program. Funding for analyzing the fish samples was provided to S.E. Hook through CSIRO Oceans and Atmosphere. Computational support was provided by the bioinformatics core at CSIRO Computational Informatics. Sequencing was provided by the Ramaciotti Centre for Gene Function Analysis at the University of New South Wales. D. Jones (formerly with CSIRO) assisted with the sample collection, H. Spiers, H. Mangs, and T. Russell of the University of New South Wales and F. Gissi (CSIRO) provided technical assistance. B. Angel, G. Batley, and P. Bain of CSIRO provided valuable discussions. A. Kumar and N. Wade (CSIRO) as well as anonymous reviewers provided insightful comments on an earlier version of the present study. The authors have no conflict of interest.
dc.description.uri http://onlinelibrary.wiley.com/doi/10.1002/etc.3505/full en_US
dc.language English
dc.language.iso en en_US
dc.relation.ispartof Null
dc.subject Differentially Regulated Genes
dc.subject Trout Oncorhynchus-mykiss
dc.subject Diuron
dc.subject Water-quality
dc.subject Imidacloprid
dc.subject Pesticide Exposure
dc.subject Environmental Sciences & Ecology
dc.subject Environmental Sciences
dc.subject Gene Expression
dc.subject Juvenile Barramundi
dc.subject Pesticides
dc.subject Platichthys-flesus
dc.subject Rna-sequence
dc.subject European Flounder
dc.subject Fathead Minnows
dc.subject Molecular-identification
dc.subject Toxicology
dc.subject Great-barrier-reef
dc.title Global transcriptomic profiling in barramundi (Lates calcarifer) from rivers impacted by differing agricultural land uses
dc.type journal article en_US
dc.identifier.doi 10.1002/etc.3505
dc.identifier.wos WOS:000391029800015


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