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Variation in heat shock protein expression at the latitudinal range limits of a widely-distributed species, the Glanville fritillary butterfly (Melitaea cinxia)

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dc.contributor World Wildlife Fund
dc.contributor Univ Texas Austin
dc.contributor University Of Texas Austin
dc.contributor Univ Plymouth
dc.contributor Dept Geol Sci
dc.contributor University Of North Carolina Chapel Hill
dc.contributor Univ North Carolina Chapel Hill
dc.contributor Dept Biol Sci
dc.contributor University Of North Carolina
dc.contributor Dept Marine Sci
dc.contributor University Of Plymouth
dc.contributor Australian Inst Marine Sci
dc.contributor Dept Integrat Biol
dc.contributor University Of Texas System
dc.contributor Australian Institute Of Marine Science MATZ, MIKHAIL V. ADVANI, NIKHIL K. KENKEL, CARLY D. DAVIES, SARAH W. PARMESAN, CAMILLE SINGER, MICHAEL C. 2017-01-13T00:45:24Z 2017-01-13T00:45:24Z 2017-03-21T01:05:00Z 2019-05-09T01:21:54Z 2017-03-21T01:05:00Z 2017-01-13T00:45:24Z 2017-01-13T00:45:24Z 2019-05-09T01:21:54Z 2016-09-01
dc.identifier.citation Advani NK, Kenkel CD, Davies SW, Parmesan C, Singer MC, Matz MV (2016) Variation in heat shock protein expression at the latitudinal range limits of a widely-distributed species, the Glanville fritillary butterfly (Melitaea cinxia). Physiological Entomology 41(3): 241-248 en_US
dc.identifier.issn 0307-6962
dc.description.abstract Studies of heat shock response show a correlation with local climate, although this is more often across altitudinal than latitudinal gradients. In the present study, differences in constitutive but not inducible components of heat shock response are detected among populations of the Glanville fritillary butterfly Melitaea cinxia L. that exist at the species' latitudinal range limits (Finland and Spain). The study demonstrates that macroclimatic differences between these sites should cause greater exposure of the Spanish population to higher temperatures. Thermal stress treatments are used to estimate differences in the expression of four genes potentially relevant for tolerating these temperatures. For the analysis, three heat-shock proteins and glyceraldehyde-3-phosphate dehydrogenase (G3PDH), a glycolysis enzyme that also modulates cell growth based on metabolic state, are chosen. Two constitutive differences are found between the sites. First, insects from Spain have higher levels of Hsp 21.4 than those from Finland regardless of thermal stress treatment; this protein is not inducible. Second, insects from Finland have higher levels of G3PDH. The two remaining Hsps, Hsp20.4 and Hsp90, show dramatic up-regulation at higher temperatures, although there are no significant differences between insects from the different populations in either constitutive levels or inducibility. In nature, differences between the study populations likely occur in the expression of all four genes that were studied, although these differences would be directly climate-induced in Hsp20.4 and Hsp90 and constitutive in Hsp21.4 and G3PDH. Inducibility may mitigate the need for constitutive variation in traits that adapt insects to local climate.
dc.description.sponsorship We thank Eli Meyer for help with primer selection and design; Galina Aglyamova and Wayne Hall for help with the laboratory experiments; James Marden and Marjo Saastamoinen for help with obtaining habitat temperature data for M. cinxia; and Constanti Stefanescu and Saskya van Nouhuys for help with collecting insects from the field. Research was supported by National Science Foundation grant DEB-1054766 to M.V.M.
dc.description.uri en_US
dc.language English
dc.language.iso en en_US
dc.publisher Wiley en_US
dc.relation.ispartof Null
dc.subject Orchesella-cincta
dc.subject Constitutive Adjustment Hypothesis
dc.subject Thermal Adaptation
dc.subject Hsp90
dc.subject Stress Resistance Traits
dc.subject Drosophila-melanogaster
dc.subject Climate Change Adaptation
dc.subject Phosphoglucose Isomerase
dc.subject Glyceraldehyde-3-phosphate Dehydrogenase
dc.subject Climate-change
dc.subject Hsp21.4
dc.subject Latitude
dc.subject Qpcr
dc.subject Genetic-variation
dc.subject Glanville Fritillary Butterfly
dc.subject Metal Tolerance
dc.subject Lycaena-tityrus
dc.subject Entomology
dc.subject Heat Shock Protein
dc.subject Hsp20.4
dc.subject Hsp70 Expression
dc.title Variation in heat shock protein expression at the latitudinal range limits of a widely-distributed species, the Glanville fritillary butterfly (Melitaea cinxia)
dc.type journal article en_US
dc.identifier.doi 10.1111/phen.12148
dc.identifier.wos WOS:000386940600008

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