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Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy

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dc.contributor Australian Institute Of Marine Science
dc.contributor Dept Chem
dc.contributor Univ Western Australia
dc.contributor Curtin University
dc.contributor Oceans Inst
dc.contributor Ctr Microscopy Characterisat & Anal
dc.contributor University Of Western Australia
dc.contributor Curtin Univ
dc.contributor Curtin Inst Funct Mol & Interfaces
dc.contributor Sch Earth Sci
dc.contributor Australian Inst Marine Sci
dc.contributor Arc Ctr Excellence Coral Reef Studies MCCULLOCH, MALCOLM T. DECARLO, THOMAS M. HOLCOMB, MICHAEL D'OLIVO, JUAN P. FOSTER, TARYN BECKER, THOMAS 2018-02-04T18:34:16Z 2018-02-04T18:34:16Z 2019-05-09T01:03:37Z 2018-02-04T18:34:16Z 2018-02-04T18:34:16Z 2019-05-09T01:03:37Z 2017-11-24
dc.identifier.citation DeCarlo TM, D'Olivo JP, Foster T, Holcomb M, Becker T, McCulloch MT (2017) Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy. Biogeosciences 14: 5253-5269
dc.identifier.issn 1726-4170
dc.description.abstract Quantifying the saturation state of aragonite (Omega(Ar)) within the calcifying fluid of corals is critical for understanding their biomineralization process and sensitivity to environmental changes including ocean acidification. Recent advances in microscopy, microprobes, and isotope geochemistry enable the determination of calcifying fluid pH and [CO32-], but direct quantification of Omega(Ar) (where Omega(Ar) D [CO32-][Ca2+]/K-sp) has proved elusive. Here we test a new technique for deriving Omega(Ar) based on Raman spectroscopy. First, we analysed abiogenic aragonite crystals precipitated under a range of Omega(Ar) from 10 to 34, and we found a strong dependence of Raman peak width on Omega(Ar) with no significant effects of other factors including pH, Mg/Ca partitioning, and temperature. Validation of our Raman technique for corals is difficult because there are presently no direct measurements of calcifying fluid Omega(Ar) available for comparison. However, Raman analysis of the international coral standard JCp-1 produced Omega(Ar) of 12.3 +/- 0.3, which we demonstrate is consistent with published skeletal Mg/Ca, Sr/Ca, B/Ca, delta B-11, and delta Ca-44 data. Raman measurements are rapid (<= 1 s), high-resolution (<= 1 mu m), precise (derived Omega(Ar) +/- 1 to 2 per spectrum depending on instrument configuration), accurate (+/- 2 if Omega(Ar) < 20), and require minimal sample preparation, making the technique well suited for testing the sensitivity of coral calcifying fluid Omega(Ar) to ocean acidification and warming using samples from natural and laboratory settings. To demonstrate this, we also show a high-resolution time series of Omega(Ar) over multiple years of growth in a Porites skeleton from the Great Barrier Reef, and we evaluate the response of Omega(Ar) in juvenile Acropora cultured under elevated CO2 and temperature.
dc.description.sponsorship For the study of the GBR coral, Harry Clarke and Kai Rankenburg at University of Western Australia provided laboratory and analytical assistance. Facilities and technical assistance for the Acropora culturing experiments were provided by the Batavia Coast Maritime Institute, with logistical and technical support from the Basile family. Frieder Klein (WHOI) assisted with the initial Raman measurements. Funding was provided by an ARC Laureate Fellowship (FL120100049) awarded to Malcolm McCulloch and the ARC Centre of Excellence for Coral Reef Studies (CE140100020). The authors acknowledge the facilities, and the scientific and technical assistance of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy, Characterisation & Analysis, The University of Western Australia, a facility funded by the University, the Western Australian State and Commonwealth governments. Jan Fietzke and one anonymous reviewer provided constructive comments that improved a previous version of this paper.
dc.language English
dc.subject Geology
dc.subject Magnesian Calcites
dc.subject Ecology
dc.subject Biogenic Carbonates
dc.subject Calcification
dc.subject Environmental Sciences & Ecology
dc.subject Ph
dc.subject Calcium Isotope Fractionation
dc.subject Anthropogenic Carbon
dc.subject Geosciences, Multidisciplinary
dc.subject Up-regulation
dc.subject Mg-calcite
dc.subject Ocean Acidification
dc.subject Scleractinian Coral
dc.title Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy
dc.type journal article
dc.identifier.doi 10.5194/bg-14-5253-2017
dc.identifier.wos WOS:000416154500001

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