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Morphological characterization of virus-like particles in coral reef sponges

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dc.contributor James Cook University
dc.contributor University Of Queensland
dc.contributor Aims Jcu
dc.contributor Univ Queensland
dc.contributor Australian Inst Marine Sci
dc.contributor Australian Ctr Ecogen
dc.contributor King Abdullah Univ Sci & Technol
dc.contributor Australian Institute Of Marine Science
dc.contributor King Abdullah University Of Science & Technology
dc.contributor Div Biol & Environm Sci & Engn
dc.contributor Kaust Environm Epigenet Program Keep
dc.contributor James Cook Univ
dc.contributor.author WEBSTER, NICOLE S.
dc.contributor.author PASCELLI, CECILIA
dc.contributor.author LAFFY, PATRICK W.
dc.contributor.author KUPRESANIN, MARIJA
dc.contributor.author RAVASI, TIMOTHY
dc.date.accessioned 2018-11-04T18:47:47Z
dc.date.accessioned 2018-11-04T18:47:47Z
dc.date.accessioned 2019-01-07T22:02:56Z
dc.date.available 2018-11-04T18:47:47Z
dc.date.available 2018-11-04T18:47:47Z
dc.date.available 2019-01-07T22:02:56Z
dc.date.issued 2018-10-17
dc.identifier.citation Pascelli C, Laffy PW, Kupresanin M, Ravasi T, Webster NS (2018) Morphological characterization of virus-like particles in coral reef sponges. Peerjl 6
dc.identifier.issn 2167-8359
dc.identifier.uri http://epubs.aims.gov.au/11068/14872
dc.description.abstract Marine sponges host complex microbial consortia that vary in their abundance, diversity and stability amongst host species. While our understanding of sponge-microbe interactions has dramatically increased over the past decade, little is known about how sponges and their microbial symbionts interact with viruses, the most abundant entities in the ocean. In this study, we employed three transmission electron microscopy (TEM) preparation methods to provide the first comprehensive morphological assessment of sponge-associated viruses. The combined approaches revealed 50 different morphologies of viral-like particles (VLPs) represented across the different sponge species. VLPs were visualized within sponge cells, within the sponge extracellular mesohyl matrix, on the sponge ectoderm and within sponge-associated microbes. Non-enveloped, non-tailed icosahedral VLPs were the most commonly observed morphotypes, although tailed bacteriophage, brick-shaped, geminate and filamentous VLPs were also detected. Visualization of sponge-associated viruses using TEM has confirmed that sponges harbor not only diverse communities of microorganisms but also diverse communities of viruses.
dc.description.sponsorship Nicole S. Webster was funded through an Australian Research Council Future Fellowship FT120100480 and Cecilia Pascelli was funded through a PhD Scholarship from The CAPES Foundation and Science without Borders. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.language English
dc.subject Vlp
dc.subject Gbr
dc.subject Science & Technology - Other Topics
dc.subject Red Sea
dc.subject Ianthella-basta
dc.subject Diversity
dc.subject Environments
dc.subject Enumerating Viruses
dc.subject Marine Viral Communities
dc.subject Multidisciplinary Sciences
dc.subject Marine Sponges
dc.subject Host-range
dc.subject Electron-microscopy
dc.subject Genomic Analysis
dc.subject Transmission Electron Microscopy
dc.subject Tem
dc.subject Scleractinian Corals
dc.subject Virus
dc.subject Great Barrier Reef
dc.subject Bacteria
dc.title Morphological characterization of virus-like particles in coral reef sponges
dc.type journal article
dc.identifier.doi 10.7717/peerj.5625
dc.identifier.wos WOS:000447542900001


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