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Transcriptome analysis provides insights into the molecular mechanisms responsible for evisceration behavior in the sea cucumber Apostichopus japonicus

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dc.contributor Australian Institute Of Marine Science
dc.contributor State Ocean Adm
dc.contributor Ctr Ocean Mega Sci
dc.contributor Lab Marine Ecol & Environm Sci
dc.contributor Inst Oceanol
dc.contributor Australian Inst Marine Sci
dc.contributor Univ Chinese Acad Sci
dc.contributor University Of Chinese Academy Of Sciences, Cas
dc.contributor State Oceanic Administration
dc.contributor Institute Of Oceanology, Cas
dc.contributor Cas Key Lab Marine Ecol & Environm Sci
dc.contributor Publ Serv Platform Industrializat Dev Technol Mar
dc.contributor Fujian Normal University
dc.contributor Chinese Acad Sci
dc.contributor Qingdao Natl Lab Marine Sci & Technol
dc.contributor University Of Chinese Academy Of Sciences
dc.contributor Institute Of Oceanology
dc.contributor Coll Life Sci
dc.contributor Chinese Academy Of Sciences
dc.contributor Fujian Normal Univ
dc.contributor.author HUANG, ZHEN
dc.contributor.author DING, KUI
dc.contributor.author ZHANG, LIBIN
dc.contributor.author SUN, LINA
dc.contributor.author LIN, CHENGGANG
dc.contributor.author FENG, QIMING
dc.contributor.author ZHANG, SHUANGYAN
dc.contributor.author YANG, HONGSHENG
dc.contributor.author BRINKMAN, RICHARD
dc.contributor.author LIN, GANG
dc.date.accessioned 2019-06-25T02:34:29Z
dc.date.accessioned 2019-06-25T02:34:29Z
dc.date.accessioned 2019-10-21T21:47:00Z
dc.date.available 2019-06-25T02:34:29Z
dc.date.available 2019-06-25T02:34:29Z
dc.date.available 2019-10-21T21:47:00Z
dc.date.issued 2019-06-01
dc.identifier.citation Ding K, Zhang LB, Sun LN, Lin CG, Feng QM, Zhang SY, Yang HS, Brinkman R, Lin G, Huang Z (2019) Transcriptome analysis provides insights into the molecular mechanisms responsible for evisceration behavior in the sea cucumber Apostichopus japonicus. Comparative Biochemistry and Physiology D-Genomics & Proteomics 30: 143-157
dc.identifier.issn 1744-117X
dc.identifier.uri http://epubs.aims.gov.au/11068/15044
dc.description.abstract The sea cucumber Apostichopus japonicus (Selenka) is a valuable economic species in Southeast Asia. It has many fascinating behavioral characteristics, such as autolysis, aestivation, regeneration, and evisceration, thus it is a notable species for studies of special behaviors. Evisceration and autotomy are controlled by the neural network and involve a complicated physiological process. The occurrence of evisceration behavior in sea cucumbers is strongly related to their environment, and it negatively impacts their economic value. Evisceration behavior plays a pivotal role in the survival of A. japonicas, and when it is induced by dramatic changes in the coastal ecological environment and the aquaculture setting it can strongly affect the economic performance of this species. Although numerous studies have focused on intestinal regeneration of A. japonicas, less is known about evisceration behavior, especially its underlying molecular mechanisms. Thus, identification of genes that regulate evisceration in the sea cucumber likely will provide a scientific explanation for this significant specific behavior. In this study, Illumina sequencing (RNA-Seq) was performed on A. japonicas specimens in three states: normal (TCQ), eviscerating (TCZ), and 3 h after evisceration (TCH). In total, 129,905 unigenes were generated with an N50 length of 2651 base pairs, and 54,787 unigenes were annotated from seven functional databases (KEGG, KOG, GO, NR, NT, Interpro, and Swiss-Prot). Additionally, 190, 191, and 320 genes were identified as differentially expressed genes (DEGs) in the comparisons of TCQ vs. TCZ, TCZ vs. TCH, and TCQ vs. TCH, respectively. These DEGs mapped to 157, 113, and 190 signaling pathways in the KEGG database, respectively. KEGG analyses also revealed that potential DEGs enriched in the categories of "environmental information processing," "organismal system," "metabolism," and "cellular processes," and they were involved in evisceration behavior in A. japonicas. These DEGs are related to muscle contraction, hormone and neurotransmitter secretion, nerve and muscle damage, energy support, cellular stress, and apoptosis. In conclusion, through our comparative analysis of A. japonicus in different stages, we identified many candidate evisceration-related genes and signaling pathways that likely are involved in evisceration behavior. These results should help further elucidate the mechanisms underlying evisceration behavior in sea cucumbers.
dc.description.sponsorship This study was supported by the China National Key Research and Development Plan Project (2016YFE0101500) (Sino-Australian Centre for Healthy Coasts), National Natural Science Foundation of China (2016YFE0101500), Funding of Youth Innovation Promotion Association, CAS (2015163), Taishan Scholars Program and the Australia China Science Research Fund (ACSRF 48162) (Sino-Australian Centre for Healthy Coasts).
dc.language English
dc.subject Holothurian Eupentacta-quinquesemita
dc.subject Matrix Metalloproteinases
dc.subject Poly(adp-ribose) Polymerase
dc.subject Genetics & Heredity
dc.subject Biochemistry & Molecular Biology
dc.subject Seasonal Evisceration
dc.subject Transcriptome
dc.subject Adenylate Kinase
dc.subject Intestine Regeneration
dc.subject Evisceration-related Genes
dc.subject Acetylcholine-receptors
dc.subject Cytochrome-c-oxidase
dc.subject Evisceration Behavior
dc.subject Dna Demethylation
dc.subject Gene-expression
dc.subject Sea Cucumber
dc.title Transcriptome analysis provides insights into the molecular mechanisms responsible for evisceration behavior in the sea cucumber Apostichopus japonicus
dc.type journal article
dc.identifier.doi 10.1016/j.cbd.2019.02.008
dc.identifier.wos WOS:000469890900014


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