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Characterization of a sponge microbiome using an integrative genome-centric approach

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dc.contributor Dept Freshwater & Marine Ecol
dc.contributor King Abdullah University Of Science & Technology
dc.contributor Australian Institute Of Marine Science
dc.contributor Australian Ctr Ecogen
dc.contributor Australian Inst Marine Sci
dc.contributor Biol & Environm Sci & Engn Div
dc.contributor Inst Biodivers & Ecosyst Dynam
dc.contributor University Of Queensland
dc.contributor Red Sea Res Ctr
dc.contributor Sch Chem & Mol Biosci
dc.contributor University Of Amsterdam
dc.contributor Univ Queensland
dc.contributor Kaust
dc.contributor Univ Amsterdam
dc.contributor.author BELL, SARA C.
dc.contributor.author ENGELBERTS, J. PAMELA
dc.contributor.author ROBBINS, STEVEN J.
dc.contributor.author ARANDA, MANUEL
dc.contributor.author WEBSTER, NICOLE S.
dc.contributor.author DE GOEIJ, JASPER M.
dc.date.accessioned 2020-02-09T18:41:24Z
dc.date.accessioned 2020-02-09T18:41:24Z
dc.date.accessioned 2020-09-02T03:58:19Z
dc.date.available 2020-02-09T18:41:24Z
dc.date.available 2020-02-09T18:41:24Z
dc.date.available 2020-09-02T03:58:19Z
dc.date.issued 2020-01-28
dc.identifier.citation Engelberts JP, Robbins SJ, de Goeij JM, Aranda M, Bell SC, Webster NS (2020) Characterization of a sponge microbiome using an integrative genome-centric approach. ISME Journal 14:1100–1110
dc.identifier.issn 1751-7362
dc.identifier.uri http://epubs.aims.gov.au/11068/15797
dc.description.abstract Marine sponges often host diverse and species-specific communities of microorganisms that are critical for host health. Previous functional genomic investigations of the sponge microbiome have focused primarily on specific symbiont lineages, which frequently make up only a small fraction of the overall community. Here, we undertook genome-centric analysis of the symbiont community in the model species Ircinia ramosa and analyzed 259 unique, high-quality metagenome-assembled genomes (MAGs) that comprised 74% of the I. ramosa microbiome. Addition of these MAGs to genome trees containing all publicly available microbial sponge symbionts increased phylogenetic diversity by 32% within the archaea and 41% within the bacteria. Metabolic reconstruction of the MAGs showed extensive redundancy across taxa for pathways involved in carbon fixation, B-vitamin synthesis, taurine metabolism, sulfite oxidation, and most steps of nitrogen metabolism. Through the acquisition of all major taxa present within the I. ramosa microbiome, we were able to analyze the functional potential of a sponge-associated microbial community in unprecedented detail. Critical functions, such as carbon fixation, which had previously only been assigned to a restricted set of sponge-associated organisms, were actually spread across diverse symbiont taxa, whereas other essential pathways, such as ammonia oxidation, were confined to specific keystone taxa.
dc.language English
dc.subject Cd-hit
dc.subject Insights
dc.subject Life-style
dc.subject Protein
dc.subject Bacterial
dc.subject Environmental Sciences & Ecology
dc.subject Symbionts
dc.subject Microbiology
dc.subject Diversity
dc.subject Marine Sponge
dc.subject Metabolism
dc.subject Reveals
dc.subject Ecology
dc.title Characterization of a sponge microbiome using an integrative genome-centric approach
dc.type journal article
dc.identifier.doi 10.1038/s41396-020-0591-9
dc.identifier.wos WOS:000509840300001


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