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Molecular techniques and their limitations shape our view of the holobiont

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dc.contributor Sch Plant Sci
dc.contributor Australian Ctr Ecogen
dc.contributor University of Kiel
dc.contributor University of Western Australia
dc.contributor National Taiwan University
dc.contributor University of Cambridge
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dc.contributor GEOMAR Helmholtz Center for Ocean Research Kiel
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dc.contributor University of Melbourne
dc.contributor.author Seemann, Torsten
dc.contributor.author Mead, Oliver
dc.contributor.author Mohamed, Amin
dc.contributor.author Cooke, Ira
dc.contributor.author Moya, Aurelie
dc.contributor.author Sakamaki, Kazuhiro
dc.contributor.author Butterfield, Nicholas J.
dc.contributor.author Robbins, Steven
dc.contributor.author Hernandez, Alejandra
dc.contributor.author Davy, Simon K.
dc.contributor.author Tang, Sen-Lin
dc.contributor.author Bosch, Thomas C. G.
dc.contributor.author Arnold, A. Elizabeth
dc.contributor.author Jaspers, Cornelia
dc.contributor.author Whalen, Casey
dc.contributor.author Pita, Lucia
dc.contributor.author Hoogenboom, Mia
dc.contributor.author Schoepf, Verena
dc.contributor.author Ragan, Mark A.
dc.contributor.author Chan, Cheong Xin
dc.contributor.author Ying, Hua
dc.contributor.author Stolarski, Jaroslaw
dc.contributor.author Cowman, Peter F.
dc.contributor.author Whitelaw, Brooke
dc.contributor.author Voolstra, Christian R.
dc.contributor.author Ball, Eldon
dc.contributor.author Miller, David
dc.contributor.author Takahashi, Shunichi
dc.contributor.author Strugnell, Jan M.
dc.contributor.author Fortunato, Sofia, V
dc.contributor.author Webster, Nicole
dc.contributor.author Boote, Chloe
dc.contributor.author Shinzato, Chuya
dc.contributor.author Andrade, Natalia R.
dc.contributor.author Darling, Aaron
dc.contributor.author Bourne, David
dc.contributor.author Ainsworth, Tracy
dc.contributor.author Adamska, Maja
dc.contributor.author Fabricius, Katharina
dc.contributor.author Fraune, Sebastian
dc.date.accessioned 2020-02-09T19:13:53Z
dc.date.accessioned 2020-09-02T03:56:49Z
dc.date.available 2020-02-09T19:13:53Z
dc.date.available 2020-09-02T03:56:49Z
dc.date.issued 2019-12-01
dc.identifier.citation Cooke I, Mead O, Whalen C, Boote C, Moya A, Ying H, Robbins S, Strugnell JM, Darling A, Miller D, Voolstra CR, Adamska M, Ainsworth T, Andrade NR, Arnold AE, Ball E, Bourne D, Bosch TCG, Butterfield NJ, Chan CX, Cowman PF, Davy SK, Fabricius K, Fortunato SV, Fraune S, Hernandez A, Hoogenboom M, Jaspers C, Mohamed A, Pita L, Ragan MA, Sakamaki K, Schoepf V, Seemann T, Shinzato C, Stolarski J, Takahashi S, Tang SL, Webster N, Whitelaw B, Consortium Australian Acad Sci Bod (2019) Molecular techniques and their limitations shape our view of the holobiont. Zoology 137:125695
dc.identifier.issn 0944-2006
dc.identifier.uri http://epubs.aims.gov.au/11068/15798
dc.description.abstract It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.
dc.description.sponsorship This review emerged from discussions on the role of molecular techniques in holobiont research that were held as part of an Australian Academy of Sciences Boden research conference on Magnetic Island in July 2018. We wish to thank organisations that funded this conference including the Australian Academy of Sciences, Great Barrier Reef Foundation, Ian Potter Foundation, the ARC CoE for coral reef studies, and the Collaborative Research Centre (CRC 1182, funded through the German Research Foundation DFG) "Origin and Function of Metaorganisms".
dc.language English
dc.subject Zoology
dc.subject Metagenomic sequencing
dc.subject BACTERIA
dc.subject ORGANIZATION
dc.subject GUT MICROBIOTA
dc.subject METAORGANISMS
dc.subject Multi-omics
dc.subject Evolutionary inference
dc.subject AIPTASIA
dc.subject IDENTIFICATION
dc.subject MICROBIAL DIVERSITY
dc.subject HORIZONTAL GENE-TRANSFER
dc.subject Holobiont
dc.subject Imaging
dc.subject Model system
dc.subject MODEL
dc.subject EXPRESSION
dc.title Molecular techniques and their limitations shape our view of the holobiont
dc.type journal article
dc.identifier.doi 10.1016/j.zool.2019.125695
dc.identifier.wos WOS:000509003500001


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