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Molecular techniques and their limitations shape our view of the holobiont

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dc.contributor Sch Plant Sci
dc.contributor Australian Ctr Ecogen
dc.contributor University of Kiel
dc.contributor University of Western Australia
dc.contributor National Taiwan University
dc.contributor University of Cambridge
dc.contributor Ctr Sustainable Trop Fisheries & Aquaculture
dc.contributor Australian Natl Univ
dc.contributor GEOMAR Helmholtz Center for Ocean Research Kiel
dc.contributor Univ Kiel
dc.contributor Australian Inst Marine Sci
dc.contributor Ctr Trop Bioinformat & Mol Biol
dc.contributor Natl Taiwan Univ
dc.contributor Australian National University
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dc.contributor Dept Ecol Environm & Evolut
dc.contributor Univ Tokyo
dc.contributor Dept Ecol & Evolutionary Biol
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dc.contributor University of Tokyo
dc.contributor James Cook University
dc.contributor University of Konstanz
dc.contributor National Institute for Basic Biology (NIBB)
dc.contributor University of Technology Sydney
dc.contributor CSIRO
dc.contributor Evolutionary Ecol Marine Fishes
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dc.contributor Univ Western Australia
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dc.contributor National Institutes of Natural Sciences (NINS) - Japan
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dc.contributor University of Melbourne Seemann, Torsten Mead, Oliver Mohamed, Amin Cooke, Ira Moya, Aurelie Sakamaki, Kazuhiro Butterfield, Nicholas J. Robbins, Steven Hernandez, Alejandra Davy, Simon K. Tang, Sen-Lin Bosch, Thomas C. G. Arnold, A. Elizabeth Jaspers, Cornelia Whalen, Casey Pita, Lucia Hoogenboom, Mia Schoepf, Verena Ragan, Mark A. Chan, Cheong Xin Ying, Hua Stolarski, Jaroslaw Cowman, Peter F. Whitelaw, Brooke Voolstra, Christian R. Ball, Eldon Miller, David Takahashi, Shunichi Strugnell, Jan M. Fortunato, Sofia, V Webster, Nicole Boote, Chloe Shinzato, Chuya Andrade, Natalia R. Darling, Aaron Bourne, David Ainsworth, Tracy Adamska, Maja Fabricius, Katharina Fraune, Sebastian 2020-02-09T19:13:53Z 2020-09-02T03:56:49Z 2020-02-09T19:13:53Z 2020-09-02T03:56:49Z 2019-12-01
dc.identifier.citation Cooke I, Mead O, Whalen C, Boote C, Moya A, Ying H, Robbins S, Strugnell JM, Darling A, Miller D, Voolstra CR, Adamska M, Ainsworth T, Andrade NR, Arnold AE, Ball E, Bourne D, Bosch TCG, Butterfield NJ, Chan CX, Cowman PF, Davy SK, Fabricius K, Fortunato SV, Fraune S, Hernandez A, Hoogenboom M, Jaspers C, Mohamed A, Pita L, Ragan MA, Sakamaki K, Schoepf V, Seemann T, Shinzato C, Stolarski J, Takahashi S, Tang SL, Webster N, Whitelaw B, Consortium Australian Acad Sci Bod (2019) Molecular techniques and their limitations shape our view of the holobiont. Zoology 137:125695
dc.identifier.issn 0944-2006
dc.description.abstract It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.
dc.description.sponsorship This review emerged from discussions on the role of molecular techniques in holobiont research that were held as part of an Australian Academy of Sciences Boden research conference on Magnetic Island in July 2018. We wish to thank organisations that funded this conference including the Australian Academy of Sciences, Great Barrier Reef Foundation, Ian Potter Foundation, the ARC CoE for coral reef studies, and the Collaborative Research Centre (CRC 1182, funded through the German Research Foundation DFG) "Origin and Function of Metaorganisms".
dc.language English
dc.subject Zoology
dc.subject Metagenomic sequencing
dc.subject BACTERIA
dc.subject Multi-omics
dc.subject Evolutionary inference
dc.subject AIPTASIA
dc.subject Holobiont
dc.subject Imaging
dc.subject Model system
dc.subject MODEL
dc.subject EXPRESSION
dc.title Molecular techniques and their limitations shape our view of the holobiont
dc.type journal article
dc.identifier.doi 10.1016/j.zool.2019.125695
dc.identifier.wos WOS:000509003500001

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