Publication Repository

A Rapid Genetic Assay for the Identification of the Most Common Pocillopora damicornis Genetic Lineages on the Great Barrier Reef

Show simple item record

dc.contributor Australian Institute Of Marine Science
dc.contributor Univ Tasmania
dc.contributor Inst Antarctic & So Ocean Studies
dc.contributor Sch Marine & Trop Biol
dc.contributor James Cook Univ
dc.contributor James Cook University
dc.contributor Great Barrier Reef Marine Pk Author
dc.contributor Aims Jcu
dc.contributor Australian Res Council Ctr Excellence Coral Reef
dc.contributor University Of Tasmania
dc.contributor Australian Inst Marine Sci
dc.contributor.author VAN OPPEN, MADELEINE J. H.
dc.contributor.author TORDA, GERGELY
dc.contributor.author SCHMIDT-ROACH, SEBASTIAN
dc.contributor.author PEPLOW, LESA M.
dc.contributor.author LUNDGREN, PETRA
dc.date.accessioned 2013-03-08T05:47:45Z
dc.date.accessioned 2017-03-21T01:07:45Z
dc.date.accessioned 2017-03-21T01:07:45Z
dc.date.accessioned 2018-11-01T03:17:37Z
dc.date.available 2013-03-08T05:47:45Z
dc.date.available 2013-03-08T05:47:45Z
dc.date.available 2017-03-21T01:07:45Z
dc.date.available 2018-11-01T03:17:37Z
dc.date.issued 2013-03-07
dc.identifier.citation Torda G, Schmidt-Roach S, Peplow LM, Lundgren P, van Oppen MJH (2013) A rapid genetic assay for the identification of the most common Pocillopora damicornis genetic lineages on the Great Barrier Reef. PLoS ONE 8(3): e58447 en_US
dc.identifier.issn 1932-6203
dc.identifier.uri http://epubs.aims.gov.au/11068/4841
dc.description.abstract Pocillopora damicornis (Linnaeus, 1758; Scleractinia, Pocilloporidae) has recently been found to comprise at least five distinct genetic lineages in Eastern Australia, some of which likely represent cryptic species. Due to similar and plastic gross morphology of these lineages, field identification is often difficult. Here we present a quick, cost effective genetic assay as well as three novel microsatellite markers that distinguish the two most common lineages found on the Great Barrier Reef. The assay is based on PCR amplification of two regions within the mitochondrial putative control region, which show consistent and easily identifiable fragment size differences for the two genetic lineages after Alu1 restriction enzyme digestion of the amplicons.
dc.description.sponsorship This work has been funded by the Australian Government's Marine and Tropical Sciences Research Fund (MTSRF), and AIMS@JCU, a collaborative scheme between the Australian Institute for Marine Science and James Cook University. G.T. is supported by the James Cook University Postgraduate Research Scholarship, and both G.T. and S.S-R. are supported by an Endeavour International Postgraduate Research Scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.description.sponsorship Marine and Tropical Sciences Research Fund (MTSRF), AIMS@JCU en_US
dc.description.uri http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0058447 en
dc.language English
dc.language.iso en en_US
dc.publisher PLoS ONE en_US
dc.relation.ispartof Null
dc.rights CC0 1.0 Universal *
dc.rights.uri http://creativecommons.org/publicdomain/zero/1.0/ *
dc.subject Pocillopora en_US
dc.subject Species Identification en_US
dc.subject Mtdna en_US
dc.subject Science & Technology - Other Topics
dc.subject Multidisciplinary Sciences
dc.subject Cnidaria
dc.subject Sequences
dc.subject Lunar Periodicity
dc.subject Morphology
dc.subject Coral
dc.subject Show
dc.subject Scleractinia
dc.subject Loci
dc.title A Rapid Genetic Assay for the Identification of the Most Common Pocillopora damicornis Genetic Lineages on the Great Barrier Reef
dc.type journal article en_US
dc.identifier.doi 10.1371/journal.pone.0058447
dc.identifier.wos WOS:000318334500084


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

CC0 1.0 Universal Except where otherwise noted, this item's license is described as CC0 1.0 Universal

Search Publication


Browse

My Account