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Transcriptional Activation of c3 and hsp70 as Part of the Immune Response of Acropora millepora to Bacterial Challenges

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dc.contributor Florida Int Univ
dc.contributor Dept Biol Sci
dc.contributor State University System Of Florida
dc.contributor Australia Inst Marine Sci
dc.contributor Florida International University
dc.contributor.author RODRIGUEZ-LANETTY, MAURICIO
dc.contributor.author BROWN, TANYA
dc.contributor.author BOURNE, DAVID
dc.date.accessioned 2017-03-21T01:07:50Z
dc.date.accessioned 2017-03-21T01:07:50Z
dc.date.accessioned 2013-07-11T04:44:08Z
dc.date.accessioned 2020-07-20T00:18:48Z
dc.date.available 2013-07-11T04:44:08Z
dc.date.available 2013-07-11T04:44:08Z
dc.date.available 2017-03-21T01:07:50Z
dc.date.available 2020-07-20T00:18:48Z
dc.date.issued 2013-07-04
dc.identifier.citation Brown T, Bourne D, Rodriguez-Lanetty M (2013) Transcriptional activation of c3 and hsp70 as part of the immune response of Acropora millepora to bacterial challenges. PLoS ONE 8(7): e67246. en_US
dc.identifier.issn 1932-6203
dc.identifier.uri http://epubs.aims.gov.au/11068/5183
dc.description.abstract The impact of disease outbreaks on coral physiology represents an increasing concern for the fitness and resilience of reef ecosystems. Predicting the tolerance of corals to disease relies on an understanding of the coral immune response to pathogenic interactions. This study explored the transcriptional response of two putative immune genes (c3 and c-type lectin) and one stress response gene (hsp70) in the reef building coral, Acropora millepora challenged for 48 hours with bacterial strains, Vibrio coralliilyticus and Alteromonas sp. at concentrations of 10(6) cells ml(-1). Coral fragments challenged with V. coralliilyticus appeared healthy while fragments challenged with Alteromonas sp. showed signs of tissue lesions after 48 hr. Coral-associated bacterial community profiles assessed using denaturing gradient gel electrophoresis changed after challenge by both bacterial strains with the Alteromonas sp. treatment demonstrating the greatest community shift. Transcriptional profiles of c3 and hsp70 increased at 24 hours and correlated with disease signs in the Alteromonas sp. treatment. The expression of hsp70 also showed a significant increase in V. coralliilyticus inoculated corals at 24 h suggesting that even in the absence of disease signs, the microbial inoculum activated a stress response in the coral. C-type lectin did not show a response to any of the bacterial treatments. Increase in gene expression of c3 and hsp70 in corals showing signs of disease indicates their potential involvement in immune and stress response to microbial challenges.
dc.description.sponsorship This research was funded by NSF-OCE grant (0851123) awarded to MRL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.description.uri http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0067246 en_US
dc.language English
dc.language.iso en en_US
dc.publisher PloS en_US
dc.relation.ispartof Null
dc.subject Science & Technology - Other Topics
dc.subject Coral Pocillopora-damicornis
dc.subject Sea-anemone
dc.subject Complement
dc.subject Scleractinian Corals
dc.subject Complement-system
dc.subject Reef Coral
dc.subject Innate Immunity
dc.subject Montastraea-faveolata
dc.subject Climate-change
dc.subject Multidisciplinary Sciences
dc.subject Artemia-franciscana
dc.subject Anemone Anemonia-viridis
dc.subject Heat-shock-protein
dc.subject Vibrio-coralliilyticus
dc.title Transcriptional Activation of c3 and hsp70 as Part of the Immune Response of Acropora millepora to Bacterial Challenges
dc.type journal article en_US
dc.identifier.doi 10.1371/journal.pone.0067246
dc.identifier.wos WOS:000323350700010


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