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Direct measurement of dimethylsulfoniopropionate (DMSP) in reef-building corals using quantitative nuclear magnetic resonance (qNMR) spectroscopy

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dc.contributor Australian Institute Of Marine Science
dc.contributor Sch Marine & Trop Biol
dc.contributor James Cook Univ
dc.contributor James Cook University
dc.contributor Aims Jcu
dc.contributor Australian Inst Marine Sci
dc.contributor Arc Ctr Excellence Coral Reef Studies
dc.contributor Sch Pharm & Mol Sci
dc.contributor.author RAINA, JEAN-BAPTISTE
dc.contributor.author LUTZ, ADRIAN
dc.contributor.author WILLIS, BETTE L.
dc.contributor.author MOTTI, CHERIE A.
dc.contributor.author TAPIOLAS, DIANNE M.
dc.date.accessioned 2017-03-21T00:55:34Z
dc.date.accessioned 2013-07-25T06:24:03Z
dc.date.accessioned 2017-03-21T00:55:34Z
dc.date.accessioned 2019-05-09T01:16:55Z
dc.date.available 2013-07-25T06:24:03Z
dc.date.available 2013-07-25T06:24:03Z
dc.date.available 2017-03-21T00:55:34Z
dc.date.available 2019-05-09T01:16:55Z
dc.date.issued 2013-05-01
dc.identifier.citation Tapiolas DM, Raina J-B, Lutz AH, Willis BL, Motti CA (2013) Direct measurement of dimethylsulfoniopropionate (DMSP) in reef-building corals using quantitative nuclear magnetic resonance (qNMR) spectroscopy. Journal of Experimental Marine Biology and Ecology 443: 85-89 en_US
dc.identifier.issn 0022-0981
dc.identifier.uri http://epubs.aims.gov.au/11068/5278
dc.description.abstract Reef building corals are among the largest producers of dimethylsulfoniopropionate (DMSP), a sulfur molecule synthesized by their endosymbiotic dinoflagellates in the genus Symbiodinium. DMSP is potentially involved in important physiological and ecological processes in corals, but investigating the functional role of this molecule requires rapid and accurate quantification techniques. Here we introduce a simple method enabling direct quantification of DMSP and one of its breakdown products acrylate using quantitative nuclear magnetic resonance (qNMR) spectroscopy. The method was tested on a range of coral genera and presents a number of advantages over currently used quantification techniques, including simultaneous and direct quantification of multiple molecules from the same extract, and rapid processing with high reproducibility enabling analyses of large numbers of samples in short time periods. The method was successfully applied to environmental samples and provides the first baseline information on diel variation of DMSP and acrylate concentrations in the coral Acropora millepora. (C) 2013 Elsevier B.V. All rights reserved.
dc.description.sponsorship We would like to thank Dr. David Abrego for collecting the different coral genera. This study was funded by the Australian Institute of Marine Science.
dc.description.uri http://www.sciencedirect.com/science/article/pii/S0022098113000981 en_US
dc.language English
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.relation.ispartof Null
dc.subject Great-barrier-reef
dc.subject Sulfur
dc.subject Algae
dc.subject Identification
dc.subject Ecology
dc.subject Quantitative Nuclear Magnetic Resonance (qnmr)
dc.subject Reef-building Corals
dc.subject Environmental Sciences & Ecology
dc.subject Climate
dc.subject Eretic Method
dc.subject Circadian Rhythm
dc.subject Marine & Freshwater Biology
dc.subject Dimethylsulfoniopropionate (dmsp)
dc.subject Odor
dc.subject Nmr
dc.subject Dimethylsulfide
dc.subject Validation
dc.title Direct measurement of dimethylsulfoniopropionate (DMSP) in reef-building corals using quantitative nuclear magnetic resonance (qNMR) spectroscopy
dc.type journal article en_US
dc.identifier.doi 10.1016/j.jembe.2013.02.037
dc.identifier.wos WOS:000320425700011


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