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Microbial diversity within the water column of a larval rearing system for the ornate rock lobster (Panulirus ornatus)

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dc.contributor University Of Queensland
dc.contributor Sch Mol & Microbial Sci
dc.contributor James Cook University
dc.contributor James Cook Univ N Queensland
dc.contributor Univ Queensland
dc.contributor Australian Inst Marine Sci
dc.contributor Australian Institute Of Marine Science
dc.contributor Sch Marine Biol & Aquaculture
dc.contributor Australian Institute Of Marine Science (aims) en
dc.contributor.author BOURNE, DAVID G.
dc.contributor.author PAYNE, MATTHEW S.
dc.contributor.author HALL, MIKE R.
dc.contributor.author BANNISTER, RAYMOND
dc.contributor.author SLY, LINDSAY
dc.date.accessioned 2013-02-28T06:50:16Z
dc.date.accessioned 2013-02-28T06:50:16Z
dc.date.accessioned 2017-03-21T01:21:41Z
dc.date.accessioned 2018-11-01T03:15:22Z
dc.date.available 2013-02-28T06:50:16Z
dc.date.available 2013-02-28T06:50:16Z
dc.date.available 2017-03-21T01:21:41Z
dc.date.available 2018-11-01T03:15:22Z
dc.date.issued 2006-08-31
dc.identifier 7195 en
dc.identifier.citation Payne M, Hall MR, Bannister R, Sly L and Bourne DG (2006) Microbial diversity within the water column of a larval rearing system for the ornate rock lobster (Panulirus ornatus). Aquaculture. 258: 80-90. en
dc.identifier.issn 0044-8486
dc.identifier.uri http://epubs.aims.gov.au/11068/7195
dc.description Link to abstract/full text - http://dx.doi.org/10.1016/j.aquaculture.2006.04.001 en
dc.description.abstract The ornate tropical rock lobster, Panulirus ornatus has substantial potential as an aquaculture species though disease outbreaks during the animal's extended larval lifecycle are major constraints for success. In order to effectively address such disease-related issues, an improved understanding of the composition and dynamics of the microbial communities in the larval rearing tanks is required. This study used flow cytometry and molecular microbial techniques (clone libraries and denaturing gradient gel electrophoresis (DGGE)) to quantify and characterise the microbial community of the water column in the early stages (developmental stage I-II) of a P. ornatus larval rearing system. DGGE analysis of a 5000 L larval rearing trial demonstrated a dynamic microbial community with distinct changes in the community structure after initial stocking (day I to day 2) and from day 4 to day 5, after which the structure was relatively stable. Flow cytometry analysis of water samples taken over the duration of the trial demonstrated a major increase in bacterial load leading up to and peaking on the first day of the initial larval moult (day 7), before markedly decreasing prior to when > 50% of larvae moulted (day 9). A clone library of a day 10 water sample taken following a mass larval mortality event reflected high microbial diversity confirmed by statistical analysis indices. Sequences retrieved from both clone library and DGGE analyses were dominated by gamma- and alpha-Proteobacteria affiliated organisms with additional sequences affiliated with beta- and epsilon-Proteobacteria, Bacteroidetes, Cytophagales and Chlamydiales groups. Vibrio affiliated species were commonly retrieved in the clone library, though absent from DGGE analysis. Crown Copyright (c) 2006 Published by Elsevier B.V. All rights reserved.
dc.description.uri http://dx.doi.org/10.1016/j.aquaculture.2006.04.001 en
dc.language English
dc.language en en
dc.relation.ispartof Aquaculture - pages: 258: 80-90 en
dc.relation.ispartof Null
dc.subject Genetic Diversity
dc.subject Polymerase-chain-reaction
dc.subject Fisheries
dc.subject Community Structure
dc.subject Flow Cytometry
dc.subject Bacterial Community
dc.subject Population
dc.subject Tiger Prawn
dc.subject Marine & Freshwater Biology
dc.subject Vibrio-harveyi
dc.subject Panulirus Ornatus Phyllosoma
dc.subject Clone Library
dc.subject Penaeus-monodon Fabricius
dc.subject 16s Ribosomal-rna
dc.subject 16s Rrna
dc.subject Gradient Gel-electrophoresis
dc.subject Dgge
dc.subject Bacterial Diversity
dc.title Microbial diversity within the water column of a larval rearing system for the ornate rock lobster (Panulirus ornatus)
dc.type journal article en
dc.identifier.doi 10.1016/j.aquaculture.2006.04.001
dc.identifier.wos WOS:000240252900007


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