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Biofilm development within a larval rearing tank of the tropical rock lobster, Panulirus ornatus

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dc.contributor Australian Institute Of Marine Science
dc.contributor Australian Inst Marine Sci
dc.contributor Australian Institute Of Marine Science (aims) en
dc.contributor.author HALL, MICHAEL R.
dc.contributor.author BOURNE, DAVID G.
dc.contributor.author HOJ, LONE
dc.contributor.author WEBSTER, NICOLE S.
dc.contributor.author SWAN, JENNIE
dc.date.accessioned 2017-03-21T00:51:18Z
dc.date.accessioned 2017-03-21T00:51:18Z
dc.date.accessioned 2013-02-28T06:45:50Z
dc.date.accessioned 2019-07-08T02:22:37Z
dc.date.available 2013-02-28T06:45:50Z
dc.date.available 2013-02-28T06:45:50Z
dc.date.available 2017-03-21T00:51:18Z
dc.date.available 2019-07-08T02:22:37Z
dc.date.issued 2006-09-29
dc.identifier 7292 en
dc.identifier.citation Bourne DG, Hoj L, Webster NS, Swan J and Hall MR (2006) Biofilm development within a larval rearing tank of the tropical rock lobster, Panulirus ornatus. Aquaculture. 260: 27-38. en
dc.identifier.issn 0044-8486
dc.identifier.uri http://epubs.aims.gov.au/11068/7292
dc.description Link to abstract/full text - http://dx.doi.org/10.1016/j.aquaculture.2006.06.016 en
dc.description.abstract The role of bacterial biofilms in disease processes is becoming increasingly recognised in both clinical and environmental settings. Biofilm development within a rearing tank of the tropical rock lobster Panulirus ornatus was studied to evaluate if the biofilm is a reservoir for potentially pathogenic bacteria that cause mass larval mortalities. Within a 5000 L larval rearing tank, fiberglass microscope slides were systematically distributed during a standard rearing attempt to assess biofilm development. Culture-based counts for two media types, TCBS and Marine Agar (MA), demonstrated increased bacterial densities until days I I and 13 respectively. For both media types, a drop in the plate counts was followed by a subsequent increase towards the end of the experiment. Scanning electron microscopy (SEM) confirmed that cell densities decreased between days 13 and 17, most likely due to sloughing of the biofilm into the water column. SEM images revealed distinct changes in dominant morphologies reflecting a succession of bacterial populations. A dynamic succession of microbial species during biofilm development was also demonstrated using denaturing gradient gel electrophoresis (DGGE) profiling of bacterial 16S rRNA genes in combination with statistical ordination analysis. Prominent changes in the DGGE profiles coincided with the decrease in bacterial numbers observed by SEM and plating on MA between days 13 and 17. Fluorescence in situ hybridization (FISH) identified alpha-Proteobacteria as being numerically abundant in the biofilm. This was supported by results from DGGE analysis, which retrieved only sequences affiliated with alpha- and gamma-Proteobacteria. DGGE bands affiliated with Fibrio became dominant towards the end of the larval run (days 21 to 24). A Fibrio harveyi strain isolated from the biofilm late in the larval rearing trial (day 24) demonstrated increased larval mortality in small scale phyllosoma survival studies. The detection of Vibrionaceae at the end of the larval trial coincided with mass phyllosoma mortality and show that the biofilm is a reservoir for potentially pathogenic bacteria. Crown Copyright (c) 2006 Published by Elsevier B.V All rights reserved.
dc.description.uri http://dx.doi.org/10.1016/j.aquaculture.2006.06.016 en
dc.language English
dc.language en en
dc.relation.ispartof Aquaculture - pages: 260: 27-38 en
dc.relation.ispartof Null
dc.relation.uri http://data.aims.gov.au/metadataviewer/uuid/28e38a61-517c-438a-ad08-7733e7f4cf79 en
dc.subject Rock Lobster
dc.subject Panulirus Ornatus
dc.subject Fisheries
dc.subject Viruses
dc.subject Genes
dc.subject Microbiology
dc.subject Biofilm Development
dc.subject Infection
dc.subject Parahaemolyticus
dc.subject Bacterial
dc.subject Marine & Freshwater Biology
dc.subject Community
dc.subject Vibrio-harveyi
dc.subject Pcr Primers
dc.subject 16s Ribosomal-rna
dc.subject Toxr
dc.title Biofilm development within a larval rearing tank of the tropical rock lobster, Panulirus ornatus
dc.type journal article en
dc.identifier.doi 10.1016/j.aquaculture.2006.06.023
dc.identifier.wos WOS:000240559700004


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