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Heterologous microarray experiments used to identify the early gene response to heat stress in a coral reef fish

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dc.contributor Adelaide Microarray Facil
dc.contributor Sch Life & Environm Sci
dc.contributor James Cook University
dc.contributor Csiro Math & Informat Sci
dc.contributor James Cook Univ N Queensland
dc.contributor Sch Trop Biol
dc.contributor Commonwealth Scientific & Industrial Research Organisation (csiro)
dc.contributor Deakin University
dc.contributor Deakin Univ
dc.contributor University Of Adelaide
dc.contributor Australian Inst Marine Sci
dc.contributor Univ Adelaide
dc.contributor Australian Institute Of Marine Science
dc.contributor Australian Institute Of Marine Science (aims) en CROZIER, ROSS H. KASSAHN, KARIN S. CALEY, M. JULIAN WARD, ALISTER C. CONNOLLY, ASHLEY R. STONE, GLENN 2013-02-28T06:44:41Z 2013-02-28T06:44:41Z 2017-03-21T00:50:49Z 2019-10-21T21:28:06Z 2017-03-21T00:50:49Z 2013-02-28T06:44:41Z 2017-03-21T00:50:49Z 2019-10-21T21:28:06Z 2007-04-01
dc.identifier 7320 en
dc.identifier.citation Kassahn KS, Caley MJ, Ward AC, Connolly A, Stone G and Crozier RH (2007) Heterologous microarray experiments used to identify the early gene response to heat stress in a coral reef fish . Molecular Ecology. 16: 1749-1763. en
dc.identifier.issn 0962-1083
dc.description Link to abstract/full text - en
dc.description.abstract Coral reef fishes are expected to experience rising sea surface temperatures due to climate change. How well tropical reef fishes will respond to these increased temperatures and which genes are important in the response to elevated temperatures is not known. Microarray technology provides a powerful tool for gene discovery studies, but the development of microarrays for individual species can be expensive and time-consuming. In this study, we tested the suitability of a Danio rerio oligonucleotide microarray for application in a species with few genomic resources, the coral reef fish Pomacentrus moluccensis. Results from a comparative genomic hybridization experiment and direct sequence comparisons indicate that for most genes there is considerable sequence similarity between the two species, suggesting that the D. rerio array is useful for genomic studies of P. moluccensis. We employed this heterologous microarray approach to characterize the early transcriptional response to heat stress in P. moluccensis. A total of 111 gene loci, many of which are involved in protein processing, transcription, and cell growth, showed significant changes in transcript abundance following exposure to elevated temperatures. Changes in transcript abundance were validated for a selection of candidate genes using quantitative real-time polymerase chain reaction. This study demonstrates that heterologous microarrays can be successfully employed to study species for which specific microarrays have not yet been developed, and so have the potential to greatly enhance the utility of microarray technology to the field of environmental and functional genomics.
dc.description.uri en
dc.language English
dc.language en en
dc.relation.ispartof Null
dc.relation.ispartof Molecular Ecology - pages: 16: 1749-1763 en
dc.subject Cdna Microarray
dc.subject Atlantic Salmon
dc.subject Ecology
dc.subject Acclimation
dc.subject Rainbow-trout
dc.subject Evolutionary Biology
dc.subject Climate-change
dc.subject Environmental Sciences & Ecology
dc.subject Heat Stress
dc.subject Cold
dc.subject Messenger-rna
dc.subject Expression Profiles
dc.subject Stress Resistance
dc.subject Hybridization
dc.subject Coral Reef Fish
dc.subject Cross-species Microarray
dc.subject Oligonucleotide Microarray
dc.subject Transcriptome
dc.subject Biochemistry & Molecular Biology
dc.title Heterologous microarray experiments used to identify the early gene response to heat stress in a coral reef fish
dc.type journal article en
dc.identifier.doi 10.1111/j.1365-294X.2006.03178.x
dc.identifier.wos WOS:000245313300015

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