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Microbial diversity within early-stage cultured Panulirus ornatus phyllosomas

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dc.contributor Australian Institute Of Marine Science
dc.contributor Sch Mol & Microbial Sci
dc.contributor University Of Queensland
dc.contributor Univ Queensland
dc.contributor Australian Inst Marine Sci
dc.contributor Australian Institute Of Marine Science (aims) en
dc.contributor.author BOURNE, DAVID G.
dc.contributor.author PAYNE, MATTHEW S.
dc.contributor.author HALL, MIKE R.
dc.contributor.author SLY, LINDSAY
dc.date.accessioned 2013-02-28T06:44:07Z
dc.date.accessioned 2017-03-21T00:49:57Z
dc.date.accessioned 2013-02-28T06:44:07Z
dc.date.accessioned 2019-05-09T01:21:52Z
dc.date.available 2013-02-28T06:44:07Z
dc.date.available 2013-02-28T06:44:07Z
dc.date.available 2017-03-21T00:49:57Z
dc.date.available 2019-05-09T01:21:52Z
dc.date.issued 2007-03-01
dc.identifier 7428 en
dc.identifier.citation Payne M, Hall MR, Sly L and Bourne DG (2007) Microbial diversity within early-stage cultured Panulirus ornatus phyllosomas. Applied and Environmental Microbiology. 73: 1940-1951. en
dc.identifier.issn 0099-2240
dc.identifier.uri http://epubs.aims.gov.au/11068/7428
dc.description Link to abstract/full text - http://dx.doi.org/10.1128/AEM.02520-06 en
dc.description.abstract A thorough understanding of the microorganisms and pathogens associated with the larval stage of the tropical ornate rock lobster, Panulirus ornatus, is required to overcome disease outbreaks that currently block aquaculture attempts. This study used microscopy in addition to culture and molecularly based microbiological techniques to characterize the bacterial community associated with cultured, developmental stage PI to II P. ornatus phyllosomas. Scanning electron microscopy demonstrated colonization of phyllosomas by filamentous, rod-shaped, and coccus-shaped bacteria. A clone library constructed from dead phyllosomas sampled from the larval rearing tank on day 10 was dominated by Thiothrix-affiliated sequences (56% of clones). A comparable library from live phyllosomas also contained Thiothrix-affiliated sequences, though these only represented 19% of clones within the library. Fluorescent in situ hybridization (FISH) confirmed identification of the filamentous bacteria as Thiothrix sp., being present on dead phyllosomas. FISH also identified Leucothrix sp. and ribrio sp., as well as a range of other rod- and coccus-shaped bacteria, colonizing both live and dead phyllosomas. The development of the microbial community associated with phyllosomas was monitored through-a standard larval rearing run using denaturing gradient gel electrophoresis (DGGE). Vibrio sp.-affiliated bands dominated the profiles of live animals through the rearing period and dead phyllosomas sampled on selected days. The population of Vibrio sp. associated with phyllosomas was monitored with culture-based analysis on selective media and demonstrated to increase significantly on day 7, coinciding with the beginning of the larval molt. An isolated Vibrio harveyi strain demonstrated an identical 16S rRNA sequence with retrieved DGGE and clone library sequences. Colonization of phyllosomas with filamentous bacterial species potentially hinders the ability of the animals to molt and, combined with the added stress of the molt process, likely results in reduced immune function, allowing opportunistic pathogenic Vibrio sp. to cause larval mortalities.
dc.description.uri http://dx.doi.org/10.1128/AEM.02520-06 en
dc.language English
dc.language en en
dc.relation.ispartof Null
dc.relation.ispartof Applied and Environmental Microbiology - pages: 73: 1940-1951 en
dc.subject Gradient Gel-electrophoresis
dc.subject Biotechnology & Applied Microbiology
dc.subject Polymerase-chain-reaction
dc.subject Activated-sludge
dc.subject Microbiology
dc.subject Phylogenetic Analysis
dc.subject Litopenaeus-vannamei
dc.subject Hypercapnic Hypoxia
dc.subject 16s Ribosomal-rna
dc.subject Luminous Vibrio-harveyi
dc.subject Tropical Rock Lobster
dc.subject Penaeus-monodon
dc.title Microbial diversity within early-stage cultured Panulirus ornatus phyllosomas
dc.type journal article en
dc.identifier.doi 10.1128/AEM.02520-06
dc.identifier.wos WOS:000245156800031


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