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Microbial diversity of mid-stage palinurid phyllosoma from great barrier reef waters

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dc.contributor Univ Bremen
dc.contributor Max Planck Inst Marine Microbiol
dc.contributor Sch Mol & Microbial Sci
dc.contributor Townsville Mail Ctr
dc.contributor University Of Queensland
dc.contributor Univ Queensland
dc.contributor Australian Inst Marine Sci
dc.contributor Max Planck Society
dc.contributor University Of Bremen
dc.contributor Australian Institute Of Marine Science
dc.contributor Australian Institute Of Marine Science (aims) en BOURNE, D. G. PAYNE, M. S. HOJ, L. WIETZ, M. HALL, M. R. SLY, L. 2017-03-21T01:25:05Z 2017-03-21T01:25:05Z 2013-02-28T06:52:00Z 2018-11-01T03:17:41Z 2017-03-21T01:25:05Z 2013-02-28T06:52:00Z 2013-02-28T06:52:00Z 2018-11-01T03:17:41Z 2008-08-01
dc.identifier 7738 en
dc.identifier.citation Payne M, Hoj L, Wietz M, Hall MR, Sly L and Bourne DG (2008) Microbial diversity of mid-stage Palinurid phyllosoma from Great Barrier Reef waters. Journal of Applied Microbiology. 105: 340-350. en
dc.identifier.issn 1364-5072
dc.description Link to abstract/full text - en
dc.description.abstract Aims: This study aimed to determine the bacterial community associated with wild-caught, mid-stage larvae of spiny lobsters (Palinuridae) in their native oligotrophic marine environment, and to compare their diversity and composition with communities associated with aquaculture-reared larvae of the tropical rock lobster Panulirus ornatus. Methods and Results: Bacterial clone libraries constructed from wild P. ornatus (two libraries) and Panulirus penicillatus (one library) larvae (phyllosoma) revealed a dominance of alpha-proteobacterial sequences, with Sulfitobacter spp.-affiliated sequences dominating both P. ornatus libraries and constituting a major portion of the P. penicillatus library. Vibrio-related sequences were rarely detected from wild phyllosoma clone libraries in contrast to similar studies of aquaculture-reared animals. Scanning electron microscopy analysis revealed low levels of bacterial colonization on the external carapace of wild phyllosoma, again in contrast to aquaculture-reared animals, which are often colonized with filamentous bacteria (mainly Thiothrix sp.) that compromise their health. Fluorescence in situ hybridization of sectioned wild phyllosoma tissue displayed low overall abundance of bacteria within the tissue and on external surfaces, with alpha-, beta-, and gamma-Proteobacteria being confirmed as members of this bacterial community. Conclusions: The consistency in predominant clone sequences retrieved from the three libraries indicated a conserved microbiota associated with wild phyllosoma. In addition, the observed differences in the microbial composition and load of reared and wild phyllosoma are indicative of the different environments in which the animals live. Significance and Impact of the Study: Bacterial disease during early larval stages is a major constraint currently hindering the development of an aquaculture industry for the ornate rock lobster P. ornatus. Knowledge of the microbial community associated with wild animals will be advantageous for the identification of bacteria that may promote animal health.
dc.description.uri en
dc.language English
dc.language en en
dc.relation.ispartof Journal of Applied Microbiology - pages: 105: 340-350 en
dc.relation.ispartof Null
dc.relation.uri en
dc.subject Rearing System
dc.subject Biotechnology & Applied Microbiology
dc.subject Scanning Electron Microscopy
dc.subject Community Structure
dc.subject Microbiology
dc.subject Hydrothermal Vent
dc.subject Phylogenetic Analysis
dc.subject Vibrio-harveyi
dc.subject Tiger Prawn
dc.subject Epsilon-proteobacteria
dc.subject Wild Phyllosoma
dc.subject Fluorescence In Situ Hybridization
dc.subject Clone Library
dc.subject 16s Rrna
dc.subject Tropical Rock Lobster
dc.subject Panulirus Sp.
dc.subject Penaeus-monodon
dc.subject Lobster Panulirus-ornatus
dc.title Microbial diversity of mid-stage palinurid phyllosoma from great barrier reef waters
dc.type journal article en
dc.identifier.doi 10.1111/j.1365-2672.2008.03749.x
dc.identifier.wos WOS:000257565400003

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