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Methods for sampling free-living Symbiodinium (zooxanthellae) and their distribution and abundance at Lizard Island (Great Barrier Reef)

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dc.contributor Australian Institute Of Marine Science
dc.contributor Sch Marine & Trop Biol
dc.contributor James Cook Univ
dc.contributor James Cook University
dc.contributor Dept Marine & Trop Biol
dc.contributor Australian Inst Marine Sci
dc.contributor Arc Ctr Excellence Coral Reef Studies
dc.contributor Australian Institute Of Marine Science (aims) en
dc.contributor.author WILLIS, BETTE L.
dc.contributor.author LITTMAN, RAECHEL A.
dc.contributor.author VAN OPPEN, MADELEINE J. H.
dc.date.accessioned 2013-02-28T06:50:38Z
dc.date.accessioned 2017-03-21T01:17:52Z
dc.date.accessioned 2017-03-21T01:17:52Z
dc.date.accessioned 2019-05-09T01:05:33Z
dc.date.available 2017-03-21T01:17:52Z
dc.date.available 2013-02-28T06:50:38Z
dc.date.available 2013-02-28T06:50:38Z
dc.date.available 2019-05-09T01:05:33Z
dc.date.issued 2008-09-12
dc.identifier 7822 en
dc.identifier.citation Littman R, van Oppen MJH and Willis BL (2008) Methods for sampling free-living Symbiodinium (zooxanthellae) and their distribution and abundance at Lizard Island (Great Barrier Reef). Journal of Experimental Marine Biology and Ecology. 364: 48-53. en
dc.identifier.issn 0022-0981
dc.identifier.uri http://epubs.aims.gov.au/11068/7822
dc.description Link to abstract/full text - http://dx.doi.org/10.1016/j.jembe.2008.06.034 en
dc.description.abstract The abundance and distribution of free-living dinoflagellates in the genus Symbiodinium have important implications for the ecology of coral reefs, determining both the symbionts available to newly recruited corals and symbiont types available for uptake by adult corals during environmental stress. However, little is known about where symbiotic dinoflagellates reside outside the host, due to the difficulty of capturing and detecting unicellular organisms in the marine environment. This study presents a successful protocol for sampling Symbiodinium from both the benthos and the water column. Comparisons of two detection methods for enumerating Symbiodinium indicated that conventional microscope analysis is accurate and more efficient when estimating Symbiodinium densities in sediment samples, while an automated particle counter (FlowCAM) was more efficient in detecting cells in the water column where densities are low. Symbiodinium densities were found to be relatively high (1000-4000 cells/mL) in sediment samples and much lower (up to 80 cells/mL) in the water column, indicating that the free-living form resides mainly in the benthos. Symbiodinium densities were found to be highly variable spatially, differing significantly between two reef locations. Within sites, elevated densities of Symbiodinium along reef margins combined with significant decreases in densities one meter away from the reef, suggest that cells aggregate within the reef habitat. (C) 2008 Elsevier B.V. All rights reserved.
dc.description.sponsorship We thank R. Berkelmans of the Australian Institute of Marine Science for access to and aid in using the FlowCAM, J. Madams and M. McCormick for support in the field, and the Australian Research Council for funding. [SS]
dc.description.uri http://dx.doi.org/10.1016/j.jembe.2008.06.034 en
dc.language English
dc.language en en
dc.relation.ispartof Null
dc.relation.ispartof Journal of Experimental Marine Biology and Ecology - pages: 364: 48-53 en
dc.subject Phytoplankton
dc.subject Microplankton
dc.subject Ecology
dc.subject Specificity
dc.subject Coral-reefs
dc.subject Diversity
dc.subject Patchiness
dc.subject Climate-change
dc.subject Environmental Sciences & Ecology
dc.subject Red-sea
dc.subject Symbiosis
dc.subject Phylogeny
dc.subject Marine & Freshwater Biology
dc.subject Zooxanthellae
dc.subject Free-living
dc.subject Symbiodinium
dc.title Methods for sampling free-living Symbiodinium (zooxanthellae) and their distribution and abundance at Lizard Island (Great Barrier Reef)
dc.type journal article en
dc.identifier.doi 10.1016/j.jembe.2008.06.034
dc.identifier.wos WOS:000260190200008


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