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Vibrio owensii sp nov., isolated from cultured crustaceans in Australia

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dc.contributor Australian Institute Of Marine Science
dc.contributor James Cook Univ
dc.contributor James Cook University
dc.contributor University Of New England
dc.contributor Aims Jcu
dc.contributor Univ New England
dc.contributor Australian Inst Marine Sci
dc.contributor Sch Sci & Technol
dc.contributor Sch Vet & Biomed Sci
dc.contributor Australian Institute Of Marine Science (aims) en
dc.contributor.author HOJ, LONE
dc.contributor.author CANO-GOMEZ, ANA
dc.contributor.author GOULDEN, EVAN F.
dc.contributor.author OWENS, LEIGH
dc.date.accessioned 2017-03-21T00:51:00Z
dc.date.accessioned 2017-03-21T00:51:00Z
dc.date.accessioned 2013-02-28T06:45:44Z
dc.date.accessioned 2019-07-08T02:09:43Z
dc.date.available 2013-02-28T06:45:44Z
dc.date.available 2013-02-28T06:45:44Z
dc.date.available 2017-03-21T00:51:00Z
dc.date.available 2019-07-08T02:09:43Z
dc.date.issued 2010-01-01
dc.identifier 8348 en
dc.identifier.citation Cano Gomez A, Goulden E, Owens L and Hoj L (2010) Vibrio owensii sp. nov., isolated from cultured crustaceans in Australia. FEMS Microbiology Letters. 302: 175-181. en
dc.identifier.issn 0378-1097
dc.identifier.uri http://epubs.aims.gov.au/11068/8348
dc.description Link to abstract/full text - http://dx.doi.org/10.1111/j.1574-6968.2009.01850.x en
dc.description.abstract Two bacterial strains (DY05T and 47666-1) were isolated in Queensland, Australia, from diseased cultured crustaceans Panulirus ornatus and Penaeus monodon, respectively. On the basis of 16S rRNA gene sequence identity, the strains were shown to belong to the Harveyi clade of the genus Vibrio. Multilocus sequence analysis using five housekeeping genes (rpoA, pyrH, topA, ftsZ and mreB) showed that the strains form a monophyletic group with 94.4% concatenated sequence identity to the closest species. DNA-DNA hybridization experiments showed that strains DY05T and 47666-1 had 76% DNA similarity to each other, but < 70% to their closest neighbours Vibrio harveyi LMG 4044T (< 55%), Vibrio campbellii LMG 11216T (< 52%) and Vibrio rotiferianus LMG 21460T (< 46%). Strains DY05T and 47666-1 could be differentiated from their relatives on the basis of several phenotypic characteristics. The major fatty acids were C(15:0) iso 2-OH and/or C(16:1) 7, C(16:0), C(18:1) 7 and C(14:0). Based on the polyphasic evidence presented here, it can be concluded that strains DY05T and 47666-1 belong to the same novel species of the genus Vibrio, for which the name Vibrio owensii sp. nov. is proposed. The type strain is DY05T (=JCM 16517T=ACM 5300T).
dc.description.sponsorship This study was funded by James Cook University, the Australian Institute of Marine Science, AIMS@JCU (Qld, Australia) and the University of New England (NSW, Australia). We thank Paul Muir (Queensland Department of Primary Industries and JCU) for isolation of strain 47666-1, and Greg Smith, Matthew Salmon and Grant Milton (AIMS) for initial sampling and plating of diseased P. ornatus larvae. We thank Linda Blackall for critically reading the manuscript.
dc.description.uri http://dx.doi.org/10.1111/j.1574-6968.2009.01850.x en
dc.language English
dc.language en en
dc.relation.ispartof FEMS Microbiology Letters - pages: 302: 175-181 en
dc.relation.ispartof Null
dc.subject Deoxyribonucleic-acid
dc.subject Vibrio Harveyi
dc.subject Vibrio Campbellii
dc.subject Microbiology
dc.subject Pathogen
dc.subject Harveyi
dc.subject Crustacean
dc.subject Vibrio Owensii
dc.subject Penaeus-monodon Larvae
dc.subject Trees
dc.subject Hybridization
dc.subject Multilocus Sequence-analysis
dc.subject Dna
dc.subject Mlsa
dc.subject Molecular-identification
dc.title Vibrio owensii sp nov., isolated from cultured crustaceans in Australia
dc.type journal article en
dc.identifier.doi 10.1111/j.1574-6968.2009.01850.x
dc.identifier.wos WOS:000272865400012


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