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Development and optimisation of ten novel microsatellite loci in three multiplex PCR panels for the Wolfie Cardinalfish (Cheilodipterus artus)

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dc.contributor Australian Institute Of Marine Science
dc.contributor University Of Western Australia
dc.contributor Australian Inst Marine Sci
dc.contributor Uwa Oceans Inst
dc.contributor Australian Institute Of Marine Science (aims) en
dc.contributor.author UNDERWOOD, JIM N.
dc.date.accessioned 2017-03-21T00:51:44Z
dc.date.accessioned 2013-02-28T06:46:01Z
dc.date.accessioned 2013-02-28T06:46:01Z
dc.date.accessioned 2018-11-01T03:06:56Z
dc.date.available 2017-03-21T00:51:44Z
dc.date.available 2017-03-21T00:51:44Z
dc.date.available 2013-02-28T06:46:01Z
dc.date.available 2018-11-01T03:06:56Z
dc.date.issued 2010-09-01
dc.identifier 8381 en
dc.identifier.citation Underwood JN (2010) Development and optimisation of ten novel microsatellite loci in three multiplex PCR panels for the Wolfie Cardinalfish (Cheilodipterus artus). Conservation Genetics Resources. 2: S261-264. en
dc.identifier.issn 1877-7252
dc.identifier.uri http://epubs.aims.gov.au/11068/8381
dc.description Link to abstract/full text - http://dx.doi.org/10.1007/s12686-010-9182-9 en
dc.description.abstract Primer pairs for ten microsatellite loci of the Wolfie Cardinalfish, Cheilodipterus artus (Apogonidae) were designed from a partial genomic DNA library. The loci were optimised and tested on 48 individuals from a single sampling site. Using three multiplex reactions, each primer pair amplified good quality, reliable and polymorphic product. The number of alleles ranged from 9 to 37 per locus and average expected heterozygosities ranged from 0.780 to 0.964. No evidence of linkage disequilibrium was detected between any pair of loci, while significant departure from Hardy-Weinberg expectation was detected at one locus only, suggesting the presence of a null allele at low frequency at this locus. These markers will be employed to measure genetic connectivity and infer influences of connectivity on population maintenance and resilience in this mouth-brooding coral reef fish that inhabits sheltered lagoons in the coral atolls of north-west of Australia.
dc.description.sponsorship Many thanks to Derek Dufall, Mike Travers, Kim Brooks, Chico Birrell, Glenn Allen, Nick Garreffa and Josh Corbett, for help with collecting the fish in the field, and to James Gilmour, Luke Smith and Madeleine van Oppen for their support in making this study happen. Also thanks to Mark Todd at Genetic Identification Services. The author acknowledges the financial support of Woodside Energy Ltd as operator of the Browse LNG Development in the conduct of this research.
dc.description.uri http://dx.doi.org/10.1007/s12686-010-9182-9 en
dc.language English
dc.language en en
dc.relation.ispartof Conservation Genetics Resources - pages: 2: S261-264 en
dc.relation.ispartof Null
dc.subject Genetics & Heredity
dc.subject Management
dc.subject Conservation
dc.subject Coral-reefs
dc.subject Connectivity
dc.subject Conservation Genetics
dc.subject Biodiversity Conservation
dc.subject Apogonidae
dc.subject Coral Reef Fish
dc.subject Biodiversity & Conservation
dc.subject Population Connectivity
dc.subject Dispersal
dc.title Development and optimisation of ten novel microsatellite loci in three multiplex PCR panels for the Wolfie Cardinalfish (Cheilodipterus artus)
dc.type journal article en
dc.identifier.doi 10.1007/s12686-010-9182-9
dc.identifier.wos WOS:000290413000062


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