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Patterns of Gene Expression in a Scleractinian Coral Undergoing Natural Bleaching

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dc.contributor Australian Institute Of Marine Science
dc.contributor Australian Natl Univ
dc.contributor James Cook Univ
dc.contributor James Cook University
dc.contributor Inst Math Sci
dc.contributor Coral Genom Grp
dc.contributor University Of Queensland
dc.contributor Australian National University
dc.contributor Ctr Marine Studies
dc.contributor Res Sch Biol Sci
dc.contributor Arc Ctr Mol Genet Dev
dc.contributor Univ Queensland
dc.contributor Australian Inst Marine Sci
dc.contributor Arc Ctr Excellence Coral Reef Studies
dc.contributor Australian Institute Of Marine Science (aims) en VAN OPPEN, MADELEINE J. H. SENECA, FRANCOIS O. FORET, SYLVAIN BALL, ELDON E. SMITH-KEUNE, CAROLYN MILLER, DAVID J. 2017-03-21T00:50:56Z 2017-03-21T00:50:56Z 2013-02-28T06:44:44Z 2019-05-09T01:17:40Z 2013-02-28T06:44:44Z 2017-03-21T00:50:56Z 2017-03-21T00:50:56Z 2019-05-09T01:17:40Z 2010-10-01
dc.identifier 8461 en
dc.identifier.citation Seneca F, Foret S, Ball EE, Smith-Keune C, Miller DJ and van Oppen MJH (2010) Patterns of gene expression in a Scleractinian coral undergoing natural bleaching. Marine Biotechnology. 12: 594-604. en
dc.identifier.issn 1436-2228
dc.description Link to abstract/full text - en
dc.description.abstract Coral bleaching is a major threat to coral reefs worldwide and is predicted to intensify with increasing global temperature. This study represents the first investigation of gene expression in an Indo-Pacific coral species undergoing natural bleaching which involved the loss of algal symbionts. Quantitative real-time polymerase chain reaction experiments were conducted to select and evaluate coral internal control genes (ICGs), and to investigate selected coral genes of interest (GOIs) for changes in gene expression in nine colonies of the scleractinian coral Acropora millepora undergoing bleaching at Magnetic Island, Great Barrier Reef, Australia. Among the six ICGs tested, glyceraldehyde 3-phosphate dehydrogenase and the ribosomal protein genes S7 and L9 exhibited the most constant expression levels between samples from healthy-looking colonies and samples from the same colonies when severely bleached a year later. These ICGs were therefore utilised for normalisation of expression data for seven selected GOIs. Of the seven GOIs, homologues of catalase, C-type lectin and chromoprotein genes were significantly up-regulated as a result of bleaching by factors of 1.81, 1.46 and 1.61 (linear mixed models analysis of variance, P < 0.05), respectively. We present these genes as potential coral bleaching response genes. In contrast, three genes, including one putative ICG, showed highly variable levels of expression between coral colonies. Potential variation in microhabitat, gene function unrelated to the stress response and individualised stress responses may influence such differences between colonies and need to be better understood when designing and interpreting future studies of gene expression in natural coral populations.
dc.description.uri en
dc.language English
dc.language en en
dc.relation.ispartof Marine Biotechnology - pages: 12: 594-604 en
dc.relation.ispartof Null
dc.subject Great-barrier-reef
dc.subject Biotechnology & Applied Microbiology
dc.subject Real-time Pcr
dc.subject Internal Control Gene
dc.subject Quantitative Pcr
dc.subject Reef-building Coral
dc.subject Inter-colony Variability
dc.subject Green Fluorescent Protein
dc.subject Thermal-stress
dc.subject Dinoflagellate Symbiodinium-microadriaticum
dc.subject Montastraea-faveolata
dc.subject Molecular Stress Response
dc.subject Gene Expression
dc.subject Marine & Freshwater Biology
dc.subject Gfp-like Proteins
dc.subject Oxidative Stress
dc.subject Symbiotic Sea-anemone
dc.subject Coral Bleaching
dc.title Patterns of Gene Expression in a Scleractinian Coral Undergoing Natural Bleaching
dc.type journal article en
dc.identifier.doi 10.1007/s10126-009-9247-5
dc.identifier.wos WOS:000282592900012

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