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Detection and Quantification of the Coral Pathogen Vibrio coralliilyticus by Real-Time PCR with TaqMan Fluorescent Probes

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dc.contributor Australian Institute Of Marine Science
dc.contributor Univ S Carolina
dc.contributor Hollings Marine Lab
dc.contributor Sch Marine & Trop Biol
dc.contributor Coll Charleston
dc.contributor James Cook Univ
dc.contributor James Cook University
dc.contributor Australian Inst Marine Sci
dc.contributor University Of South Carolina
dc.contributor Arc Ctr Excellence Coral Reef Studies
dc.contributor College Of Charleston
dc.contributor Baruch Inst Marine & Coastal Sci
dc.contributor Australian Institute Of Marine Science (aims) en
dc.contributor.author BOURNE, DAVID G.
dc.contributor.author POLLOCK, F. JOSEPH
dc.contributor.author MORRIS, PAMELA J.
dc.contributor.author WILLIS, BETTE L.
dc.date.accessioned 2013-02-28T06:47:11Z
dc.date.accessioned 2013-02-28T06:47:11Z
dc.date.accessioned 2017-03-21T01:11:58Z
dc.date.accessioned 2019-05-09T01:20:57Z
dc.date.available 2017-03-21T01:11:58Z
dc.date.available 2017-03-21T01:11:58Z
dc.date.available 2013-02-28T06:47:11Z
dc.date.available 2019-05-09T01:20:57Z
dc.date.issued 2010-08-01
dc.identifier 8521 en
dc.identifier.citation Pollock FJ, Morris PJ, Willis BL and Bourne DG (2010) Detection and quantification of the coral pathogen Vibrio coralliilyticus by use of real-time PCR with TaqMan fluorescent probes. Applied and Environmental Microbiology. 76: 5282-5286. en
dc.identifier.issn 0099-2240
dc.identifier.uri http://epubs.aims.gov.au/11068/8521
dc.description Link to abstract/full text - http://dx.doi.org/10.1128/AEM.00330-10 en
dc.description.abstract A real-time quantitative PCR-based detection assay targeting the dnaJ gene ( encoding heat shock protein 40) of the coral pathogen Vibrio coralliilyticus was developed. The assay is sensitive, detecting as little as 1 CFU per ml in seawater and 104 CFU per cm(2) of coral tissue. Moreover, inhibition by DNA and cells derived from bacteria other than V. coralliilyticus was minimal. This assay represents a novel approach to coral disease diagnosis that will advance the field of coral disease research.
dc.description.sponsorship This work was supported in part by an Australian Research Council DP to B. L. Willis, a National Science Foundation Biodiversity Surveys and Inventories Grant (DEB0516347) to P. J. Morris, and a Fulbright Postgraduate Fellowship to F.J. Pollock.
dc.description.uri http://dx.doi.org/10.1128/AEM.00330-10 en
dc.language English
dc.language en en
dc.relation.ispartof Applied and Environmental Microbiology - pages: 76: 5282-5286 en
dc.relation.ispartof Null
dc.subject Temperature
dc.subject Biotechnology & Applied Microbiology
dc.subject Microbiology
dc.subject Pocillopora-damicornis
dc.subject Shiloi
dc.subject Tissue
dc.title Detection and Quantification of the Coral Pathogen Vibrio coralliilyticus by Real-Time PCR with TaqMan Fluorescent Probes
dc.type journal article en
dc.identifier.doi 10.1128/AEM.00330-10
dc.identifier.wos WOS:000280266200043


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