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Microbial diversity in marine biofilms along a water quality gradient on the Great Barrier Reef

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dc.contributor Australian Institute Of Marine Science
dc.contributor Max Planck Inst Marine Microbiol
dc.contributor Australian Inst Marine Sci
dc.contributor Max Planck Society
dc.contributor Australian Institute Of Marine Science (aims) en
dc.contributor.author UTHICKE, SVEN
dc.contributor.author KRIWY, PASCAL
dc.date.accessioned 2017-03-21T01:23:03Z
dc.date.accessioned 2017-03-21T01:23:03Z
dc.date.accessioned 2013-02-28T06:51:06Z
dc.date.accessioned 2019-05-09T01:10:22Z
dc.date.available 2017-03-21T01:23:03Z
dc.date.available 2013-02-28T06:51:06Z
dc.date.available 2013-02-28T06:51:06Z
dc.date.available 2019-05-09T01:10:22Z
dc.date.issued 2011-04-01
dc.identifier 8743 en
dc.identifier.citation Kriwy P and Uthicke S (2011) Microbial diversity in marine biofilms along a water quality gradient on the Great Barrier Reef. Systematic and Applied Microbiology. 34: 116-126. en
dc.identifier.issn 0723-2020
dc.identifier.uri http://epubs.aims.gov.au/11068/8743
dc.description Link to abstract/full text - http://dx.doi.org/10.1016/j.syapm.2011.01.003 en
dc.description.abstract Microbial communities are potential indicators for water quality as they respond rapidly to environmental changes. In the Whitsunday Islands, Australia, microbial biofilm communities from two offshore islands were compared to those from two inshore islands subjected to poor water quality. Biofilm community composition was characterized using three culture-independent molecular techniques. The clone libraries indicated high genetic diversity, with somewhat higher scores in the offshore sites (57%) compared to the inshore sites (41%). The majority of microbes in the biofilms were related to Alphaproteobacteria (39.8%), Gammaproteobacteria (14.1%), Bacteroidetes (13.2%), diatoms (8.3%) and Cyanobacteria (3.9%). Redundancy analysis (RDA) for the CARD-FISH data showed distinct microbial assemblages between offshore and inshore communities. Additionally, 5 out of 13 water quality parameters (DIN, Chla, POP, TSS and POC) explained a significant amount of variation in the microbial communities and high values of these were associated with inshore communities. Analysis of variance (ANOVA) indicated that Cyanobacteria (p = 0.01), Bacteroidetes (p = 0.04) and to some extent Alphaproteobacteria (p = 0.07), were significantly more abundant in the offshore biofilm communities. Principal Component Analysis (PCA) of DGGE data showed clear grouping of cyanobacterial communities into inshore and offshore communities. Reasons for community shifts in the bacterial lineages are currently not resolved. One possible causative factor may be that autotrophic primary producers are more dominant in offshore sites due to the higher light availability as well as the limitation by DIN. The trends found in this study are the bases for more detailed research on microbial indicator species for changes in water quality. (C) 2011 Elsevier GmbH. All rights reserved.
dc.description.sponsorship We wish to thank the crew and masters of the RV Cape Ferguson for assisting with field work carried out in this project, J. Wulf (Max Planck Institute for Marine Microbiology, Bremen, Germany) for assistance in the laboratory work and V. Witt (Australian Institute of Marine Science, Townsville, Australia) and C. jessen (Leibniz Center for Tropical Marine Ecology, Bremen, Germany) for reviewing. Special thanks to the MarMic educational programme (Max Planck Society) for funding this project. This research project was also supported by the Australian Government's Marine and Tropical Sciences Research Facility, implemented in North Queensland by the Reef and Rainforest Research Centre Ltd.
dc.description.uri http://dx.doi.org/10.1016/j.syapm.2011.01.003 en
dc.language English
dc.language en en
dc.relation.ispartof Systematic and Applied Microbiology - pages: 34: 116-126 en
dc.relation.ispartof Null
dc.subject 16s Rdna Fragments
dc.subject Biofilms
dc.subject Biotechnology & Applied Microbiology
dc.subject Environmental Impact
dc.subject Artificial Surfaces
dc.subject Microbiology
dc.subject Microbial Community Shift
dc.subject Gel-electrophoresis
dc.subject Sequence Data
dc.subject Terrestrial Runoff
dc.subject In-situ Hybridization
dc.subject Ribosomal-rna Genes
dc.subject Targeted Oligonucleotide Probes
dc.subject Coral
dc.subject Artificial Substrates
dc.subject Sp-nov
dc.subject Water Quality Gradient
dc.subject Bacterial Communities
dc.title Microbial diversity in marine biofilms along a water quality gradient on the Great Barrier Reef
dc.type journal article en
dc.identifier.doi 10.1016/j.syapm.2011.01.003
dc.identifier.wos WOS:000289330100005


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