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Strict thermal threshold identified by quantitative PCR in the sponge Rhopaloeides odorabile

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dc.contributor Australian Institute Of Marine Science
dc.contributor Australian Inst Marine Sci
dc.contributor Australian Institute Of Marine Science (aims) en WEBSTER, NICOLE PANTILE, RAFFAELLA 2017-03-21T01:10:13Z 2013-02-28T06:52:48Z 2013-02-28T06:52:48Z 2019-10-21T21:30:13Z 2013-02-28T06:52:48Z 2017-03-21T01:10:13Z 2017-03-21T01:10:13Z 2019-10-21T21:30:13Z 2011-01-01
dc.identifier 8865 en
dc.identifier.citation Pantile R and Webster NS (2011) Strict thermal threshold identified by quantitative PCR in the sponge Rhopaloeides odorabile. Marine Ecology Progress Series. 431: 97-105. en
dc.identifier.issn 0171-8630
dc.description Link to abstract/full text - en
dc.description.abstract In light of increasing sea surface temperatures, quantifying the expression of stress-inducible genes in coastal organisms is imperative to identify early biomarkers of thermal stress. In the present study we developed a quantitative PCR (qPCR) assay to test the molecular response to heat stress in the Great Barrier Reef sponge Rhopaloeides odorabile. Suitable reference genes (coding for a-tubulin, 28S rRNA and ubiquitin) were identified among 5 candidates and then used to normalise expression of target genes (actin-related protein, calmodulin, ferritin, ubiquitin-conjugating enzyme, heat shock protein 90 [Hsp90] and heat shock protein 40 [Hsp40]) in samples exposed to high temperatures (31 and 32 degrees C) for 1, 3, 14 and 15 d. A rapid down-regulation of most genes (actin-related protein, ferritin, calmodulin and Hsp90) was observed at both temperatures within 24 h, indicating an initial shut-down of the sponge's molecular systems in response to thermal stress. The increased expression of Hsp40 and Hsp90 in sponges at 32 degrees C after 1 and 3 d respectively indicates an activation of the heat shock response system and is consistent with their role as chaperones for directing degraded proteins to proteolysis, this last process being sustained by an induction of the ubiquitin-conjugating enzyme gene at this temperature. While sponges kept at 32 degrees C only survived for the first 3 d, none of the genes in sponges kept at 31 degrees C were significantly different from those in the 27 degrees C controls after 14 d. This indicates a very strict thermal threshold for R. odorabile between 31 and 32 degrees C and is consistent with previous findings based on sponge necrosis and symbiotic disruptions in this species.
dc.description.uri en
dc.language English
dc.language en en
dc.relation.ispartof Marine Ecology Progress Series - pages: 431: 97-105 en
dc.relation.ispartof Null
dc.subject Rt-pcr
dc.subject Real-time Pcr
dc.subject Ecology
dc.subject Quantitative Pcr
dc.subject Coral-reefs
dc.subject Heat-shock-protein
dc.subject Saccharomyces-cerevisiae
dc.subject Housekeeping Gene Selection
dc.subject Climate-change
dc.subject Environmental Sciences & Ecology
dc.subject Qpcr
dc.subject Polymerase Chain-reaction
dc.subject Porifera
dc.subject Marine & Freshwater Biology
dc.subject Suberites-domuncula
dc.subject Oceanography
dc.subject Thermal Stress
dc.subject Oxidative Stress
dc.subject Great Barrier Reef
dc.title Strict thermal threshold identified by quantitative PCR in the sponge Rhopaloeides odorabile
dc.type journal article en
dc.identifier.doi 10.3354/meps09128
dc.identifier.wos WOS:000291953100008

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